Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin Immobilized on Silver Nanoparticles

Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin... ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 5, pp. 514–517. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 5, pp. 460–464. Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin Immobilized on Silver Nanoparticles a, b a a, b, A. V. Plyushchenko , L. N. Borovikova , and O. A. Pisarev * Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, 199004 Russia Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia *e-mail: chrom79@yandex.ru Received January 16, 2019; revised March 20, 2019; accepted April 22, 2019 Abstract—Two methods for the synthesis of hybrid organic-inorganic nanocomplexes comprising chymo- trypsin and silver nanoparticles have been developed based on the chemical reduction of silver nitrate by sodium borohydride. In the first method, the enzyme is added to the reaction mixture simultaneously with other reagents. In the second method, it is added to the reaction mixture at certain intervals after the start of the reduction reaction. Chymotrypsin immobilized on nanocomplexes stabilizes silver nanoparticles. As compared to the native enzyme, the immobilized enzyme prepared by the both methods exhibits an increased http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin Immobilized on Silver Nanoparticles

Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/effect-of-the-method-of-nanocomplex-synthesis-on-the-proteolytic-0u2o05Qw9L

References (8)

Publisher
Springer Journals
Copyright
Copyright © 2019 by Pleiades Publishing, Inc.
Subject
Life Sciences; Biochemistry, general; Microbiology; Medical Microbiology
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683819050090
Publisher site
See Article on Publisher Site

Abstract

ISSN 0003-6838, Applied Biochemistry and Microbiology, 2019, Vol. 55, No. 5, pp. 514–517. © Pleiades Publishing, Inc., 2019. Russian Text © The Author(s), 2019, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2019, Vol. 55, No. 5, pp. 460–464. Effect of the Method of Nanocomplex Synthesis on the Proteolytic Activity of Chymotrypsin Immobilized on Silver Nanoparticles a, b a a, b, A. V. Plyushchenko , L. N. Borovikova , and O. A. Pisarev * Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, 199004 Russia Peter the Great St. Petersburg Polytechnic University, St. Petersburg, 195251 Russia *e-mail: chrom79@yandex.ru Received January 16, 2019; revised March 20, 2019; accepted April 22, 2019 Abstract—Two methods for the synthesis of hybrid organic-inorganic nanocomplexes comprising chymo- trypsin and silver nanoparticles have been developed based on the chemical reduction of silver nitrate by sodium borohydride. In the first method, the enzyme is added to the reaction mixture simultaneously with other reagents. In the second method, it is added to the reaction mixture at certain intervals after the start of the reduction reaction. Chymotrypsin immobilized on nanocomplexes stabilizes silver nanoparticles. As compared to the native enzyme, the immobilized enzyme prepared by the both methods exhibits an increased

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Sep 25, 2019

There are no references for this article.