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Effect of small doses of insulin in vivo on the proliferation and cellularity of adipose tissue

Effect of small doses of insulin in vivo on the proliferation and cellularity of adipose tissue 125 10 10 1 1 L. Kazdová P. Fábry A. Vrána Research Centre of Metabolism and Nutrition of the Institute for Clinical and Experimental Medicine Prague Czechoslovakia Summary The effect of insulin in vivo on the proliferation and cellularity of epididymal adipose tissue of growing rats was investigated. Following the intraperitoneal administration of small amounts of insulin (500 μU/ rat, twice a day), which did not influence the blood sugar level and food intake, it was found that repeated administration of insulin for 48–72 h leads in adipose tissue to an enhanced incorporation of 14 C-2-thymidine into DNA and to an increase of the total amount of DNA and RNA in the fat body. The enhanced DNA synthesis in adipose tissue of insulin-treated rats was marked in nuclear DNA and absent in mitochondrial DNA. After fractionation of adipose tissue by collagenase an enhanced DNA synthesis was found in the fraction of adipose and stromavascular cells. Morphological examination of adipose tissue of insulin-treated rats revealed cells in the phase of mitotic division and an increased ratio of fat cells of smaller size. Calculation of the number of cells in the epididymal fat body revealed that, after administration of insulin, the number of adipose and stromavascular cells increased. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetologia Springer Journals

Effect of small doses of insulin in vivo on the proliferation and cellularity of adipose tissue

Diabetologia , Volume 10 (1) – Feb 1, 1974

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References (64)

Publisher
Springer Journals
Copyright
Copyright © 1974 by Springer-Verlag
Subject
Medicine & Public Health; Human Physiology; Internal Medicine; Metabolic Diseases
ISSN
0012-186X
eISSN
1432-0428
DOI
10.1007/BF00421417
Publisher site
See Article on Publisher Site

Abstract

125 10 10 1 1 L. Kazdová P. Fábry A. Vrána Research Centre of Metabolism and Nutrition of the Institute for Clinical and Experimental Medicine Prague Czechoslovakia Summary The effect of insulin in vivo on the proliferation and cellularity of epididymal adipose tissue of growing rats was investigated. Following the intraperitoneal administration of small amounts of insulin (500 μU/ rat, twice a day), which did not influence the blood sugar level and food intake, it was found that repeated administration of insulin for 48–72 h leads in adipose tissue to an enhanced incorporation of 14 C-2-thymidine into DNA and to an increase of the total amount of DNA and RNA in the fat body. The enhanced DNA synthesis in adipose tissue of insulin-treated rats was marked in nuclear DNA and absent in mitochondrial DNA. After fractionation of adipose tissue by collagenase an enhanced DNA synthesis was found in the fraction of adipose and stromavascular cells. Morphological examination of adipose tissue of insulin-treated rats revealed cells in the phase of mitotic division and an increased ratio of fat cells of smaller size. Calculation of the number of cells in the epididymal fat body revealed that, after administration of insulin, the number of adipose and stromavascular cells increased.

Journal

DiabetologiaSpringer Journals

Published: Feb 1, 1974

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