Ds insertion mutagenesis as an efficient tool to produce diverse variations for rice breeding

Ds insertion mutagenesis as an efficient tool to produce diverse variations for rice breeding The availability of diversified germplasm resources is the most important for developing improved rice varieties with higher seed yield or tolerance to various biotic or abiotic stresses. Here we report an efficient tool to create increased variations in rice by maize Ac/Ds transposon (a gene trap system) insertion mutagenesis. We have generated around 20,000 Ds insertion rice lines of which majority are homozygous for Ds element. We subjected these lines to phenotypic and abiotic stress screens and evaluated these lines with respect to their seed yields and other agronomic traits as well as their tolerance to drought, salinity and cold. Based on this evaluation, we observed that random Ds insertions into rice genome have led to diverse variations including a range of morphological and conditional phenotypes. Such differences in phenotype among these lines were accompanied by differential gene expression revealed by GUS histochemical staining of gene trapped lines. Among the various phenotypes identified, some Ds lines showed significantly higher grain yield compared to wild-type plants under normal growth conditions indicating that rice could be improved in grain yield by disrupting certain endogenous genes. In addition, several 1,000s of Ds lines were subjected to abiotic stresses to identify conditional mutants. Subsequent to these screens, over 800 lines responsive to drought, salinity or cold stress were obtained, suggesting that rice has the genetic potential to survive under abiotic stresses when appropriate endogenous genes were suppressed. The mutant lines that have higher seed yielding potential or display higher tolerance to abiotic stresses may be used for rice breeding by conventional backcrossing combining with molecular marker-assisted selection. In addition, by exploiting the behavior of Ds to leave footprints upon remobilization, we have shown an alternative strategy to develop new rice varieties without foreign DNA sequences in their genome. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Ds insertion mutagenesis as an efficient tool to produce diverse variations for rice breeding

18 pages
Read Article

Loading next page...
 
/lp/springer-journals/ds-insertion-mutagenesis-as-an-efficient-tool-to-produce-diverse-UTUEbEaCrT
Publisher
Springer Journals
Copyright
Copyright © 2007 by Springer Science+Business Media B.V.
Subject
Life Sciences; Plant Pathology; Biochemistry, general; Plant Sciences
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-007-9233-0
Publisher site
See Article on Publisher Site

Abstract

The availability of diversified germplasm resources is the most important for developing improved rice varieties with higher seed yield or tolerance to various biotic or abiotic stresses. Here we report an efficient tool to create increased variations in rice by maize Ac/Ds transposon (a gene trap system) insertion mutagenesis. We have generated around 20,000 Ds insertion rice lines of which majority are homozygous for Ds element. We subjected these lines to phenotypic and abiotic stress screens and evaluated these lines with respect to their seed yields and other agronomic traits as well as their tolerance to drought, salinity and cold. Based on this evaluation, we observed that random Ds insertions into rice genome have led to diverse variations including a range of morphological and conditional phenotypes. Such differences in phenotype among these lines were accompanied by differential gene expression revealed by GUS histochemical staining of gene trapped lines. Among the various phenotypes identified, some Ds lines showed significantly higher grain yield compared to wild-type plants under normal growth conditions indicating that rice could be improved in grain yield by disrupting certain endogenous genes. In addition, several 1,000s of Ds lines were subjected to abiotic stresses to identify conditional mutants. Subsequent to these screens, over 800 lines responsive to drought, salinity or cold stress were obtained, suggesting that rice has the genetic potential to survive under abiotic stresses when appropriate endogenous genes were suppressed. The mutant lines that have higher seed yielding potential or display higher tolerance to abiotic stresses may be used for rice breeding by conventional backcrossing combining with molecular marker-assisted selection. In addition, by exploiting the behavior of Ds to leave footprints upon remobilization, we have shown an alternative strategy to develop new rice varieties without foreign DNA sequences in their genome.

Journal

Plant Molecular Biology – Springer Journals

Published: Sep 19, 2007

References

  • Global impact of mutation-derived varieties
    Ahloowalia, BS; Maluszynski, M; Nichterlein, K
  • Salt tolerance conferred by over-expression of a vacuolar Na+/H+ antiport in Arabidopsis
    Apse, MP; Aharon, GS; Snedden, WA; Blumwald, E
  • Vacuolar cation/H+ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na+/H+ antiporter
    Apse, MP; Sottosanto, JB; Blumwald, E
  • Recent advances in rice biotechnology—towards genetically superior transgenic rice
    Bajaj, S; Mohanty, A
  • Biochemistry and molecular biology of plants
    Bray, EA; Bailey-Serres, J; Weretilnyk, E
  • Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays
    Brenner, S; Johnson, M; Bridgham, J; Golda, G; Lloyd, DH; Johnson, D; Luo, S; McCurdy, S; Foy, M; Ewan, M; Roth, R; George, D; Eletr, S; Albrecht, G; Vermaas, E; Williams, SR; Moon, K; Burcham, T; Pallas, M; DuBridge, RB; Kirchner, J; Fearon, K; Mao, J; Corcoran, K
  • Prospects and perspectives in mutation breeding
    Brock, RD
  • A plant DNA minipreparation, version 2
    Dellaporta, SL; Wood, J; Hicks, JB
  • The molecular genetics of crop domestication
    Doebley, JF; Gaut, BS; Smith, BD
  • Ac as a tool for the functional genomics of rice
    Enoki, H; Izawa, T; Kawahara, M; Komatsu, M; Koh, S; Kyozuka, J; Shimamoto, K
  • Assessing the impact of the green revolution, 1960 to 2000
    Evenson, RE; Gollin, D
  • AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis
    Fujita, Y; Fujita, M; Satoh, R; Maruyama, K; Parvez, MM; Seki, M; Hiratsu, K; Ohme-Takagi, M; Shinozaki, K; Yamaguchi-Shinozaki, K
  • Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis
    Greco, R; Ouwerkerk, PB; Taal, AJ; Favalli, C; Beguiristain, T; Puigdomenech, P; Colombo, L; Hoge, JH; Pereira, A
  • Unused natural variation can lift yield barriers in plant breeding
    Gur, A; Zamir, D
  • Rice mutant resources for gene discovery
    Hirochika, H; Guiderdoni, E; An, G; Hsing, YI; Eun, MY; Han, CD; Upadhyaya, N; Ramachandran, S; Zhang, Q; Pereira, A; Sundaresan, V; Leung, H
  • A rice gene activation/knockout mutant resource for high throughput functional genomics
    Hsing, YI; Chern, CG; Fan, MJ; Lu, PC; Chen, KT; Lo, SF; Sun, PK; Ho, SL; Lee, KW; Wang, YC; Huang, WL; Ko, SS; Chen, S; Chen, JL; Chung, CI; Lin, YC; Hour, AL; Wang, YW; Chang, YC; Tsai, MW; Lin, YS; Chen, YC; Yen, HM; Li, CP; Wey, CK; Tseng, CS; Lai, MH; Huang, SC; Chen, LJ; Yu, SM
  • An mRNA cap binding protein, ABH1, modulates early abscisic acid signal transduction in Arabidopsis
    Hugouvieux, V; Kwak, JM; Schroeder, JI
  • Transposon tagging in rice
    Izawa, T; Ohnishi, T; Nakano, T; Ishida, N; Enoki, H; Hashimoto, H; Itoh, K; Terada, R; Wu, C; Miyazaki, C; Endo, T; Iida, S; Shimamoto, K
  • Tissue culture-derived variation in crop improvement
    Jain, SM
  • Somaclonal variation and induced mutations in crop improvement
    Jain, SM; Brar, DS; Ahloowalia, BS
  • Assaying chimeric genes in plants, the GUS gene fusion system
    Jefferson, RA
  • Generation of a flanking sequence-tag database for activation-tagging lines in japonica rice
    Jeong, DH; An, S; Park, S; Kang, HG; Park, GG; Kim, SR; Sim, J; Kim, YO; Kim, MK; Kim, SR; Kim, J; Shin, M; Jung, M; An, G
  • The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein
    Jiang, SY; Cai, M; Ramachandran, S
  • ORYZA SATIVA MYOSIN XI B controls pollen development by photoperiod-sensitive protein localizations
    Jiang, SY; Cai, M; Ramachandran, S
  • Rice Undeveloped Tapetum1 is a major regulator of early tapetum development
    Jung, KH; Han, MJ; Lee, YS; Kim, YW; Hwang, I; Kim, MJ; Kim, YK; Nahm, BH; An, G
  • Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development
    Jung, KH; Han, MJ; Lee, DY; Lee, YS; Schreiber, L; Franke, R; Faust, A; Yephremov, A; Saedler, H; Kim, YW; Hwang, I; An, G
  • Establishing an efficient Ac/Ds tagging system in rice: large-scale analysis of Ds flanking sequences
    Kolesnik, T; Szeverenyi, I; Bachmann, D; Kumar, CS; Jiang, SY; Ramamoorthy, R; Cai, M; Ma, ZG; Sundaresan, V; Ramachandran, S
  • Origin, dispersal, cultivation and variation of rice
    Khush, GS
  • What it will take to feed 5.0 billion rice consumers in 2030
    Khush, GS
  • Rapid, large-scale generation of Ds transposant lines and analysis of the Ds insertion sites in rice
    Kim, CM; Piao, HL; Park, SJ; Chon, NS; Je, BI; Sun, B; Park, SH; Park, JY; Lee, EJ; Kim, MJ; Chung, WS; Lee, KH; Lee, YS; Lee, JJ; Won, YJ; Yi, G; Nam, MH; Cha, YS; Yun, DW; Eun, MY; Han, CD
  • Efficient insertional mutagenesis in rice using the maize En/Spm elements
    Kumar, CS; Wing, RA; Sundaresan, V
  • Somaclonal variation—a novel source of variability from cell cultures for plant improvement
    Larkin, PJ; Scowcroft, WR
  • Anatomy and cytology of microsporogenesis in cytoplasmic male sterile angiosperms
    Laser, KD; Lersten, NR
  • Thermal asymmetric interlaced PCR, automatable amplication and sequencing of insert end fragments from P1 and YAC clones for chromosome walking
    Liu, YG; Whittier, RF
  • A novel drought-inducible gene, ATAF1, encodes a NAC family protein that negatively regulates the expression of stress-responsive genes in Arabidopsis
    Lu, PL; Chen, NZ; An, R; Su, Z; Qi, BS; Ren, F; Chen, J; Wang, XC
  • Cold, salinity and drought stresses: an overview
    Mahajan, S; Tuteja, N
  • Diversifying selection in plant breeding
    McCouch, S
  • Large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
    Miyao, A; Iwasaki, Y; Kitano, H; Itoh, J; Maekawa, M; Murata, K; Yatou, O; Nagato, Y; Hirochika, H
  • Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth
    Oh, SJ; Song, SI; Kim, YS; Jang, HJ; Kim, SY; Kim, M; Kim, YK; Nahm, BH; Kim, JK
  • A mutation in the Cap Binding Protein 20 gene confers drought tolerance to Arabidopsis
    Papp, I; Mur, LA; Dalmadi, A; Dulai, S; Koncz, C
  • Transposons as tools for functional genomics
    Ramachandran, S; Sundaresan, V
  • Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways
    Rodriguez Milla, MA; Maurer, A; Rodriguez Huete, A; Gustafson, JP
  • A study of pollen viability and longevity in Oryza rufipogon, O sativa, and their hybrids
    Song, ZP; Lu, BR; Chen, JK
  • Regulation of plant disease resistance, stress responses, cell death, and ethylene signaling in Arabidopsis by the EDR1 protein kinase
    Tang, D; Christiansen, KM; Innes, RW
  • Rice functional genomics—challenges, progress and prospects
  • Two-line hybrid rice breeding manual
    Virmani, SS; Sun, ZX; Mou, TM; Jauhar Ali, A; Mao, CX
  • Towards molecular breeding and improvement of rice in China
    Wang, Y; Xue, Y; Li, J
  • Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase
    Xiong, L; Yang, Y
  • Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses
    Yamaguchi-Shinozaki, K; Shinozaki, K
  • Hybrid rice technology for food security in the world
    Yuan, L
  • Global genome expression analysis of rice in response to drought and high-salinity stresses in shoot, flag leaf, and panicle
    Zhou, J; Wang, X; Jiao, Y; Qin, Y; Liu, X; He, K; Chen, C; Ma, L; Wang, J; Xiong, L; Zhang, Q; Fan, L; Deng, XW
  • Plant salt tolerance
    Zhu, JK
  • The ANTHER INDEHISCENCE1 gene encoding a single MYB domain protein is involved in anther development in rice
    Zhu, QH; Ramm, K; Shivakkumar, R; Dennis, ES; Upadhyaya, NM

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial