DNA degradation in fresh chicken was studied with the neutral comet assay. Chicken legs were stored in a refrigerator at 2–4 °C. DNA from muscle tissue was analysed independently by the authors in Mol and Uppsala after 1–12 days. The cells were imbedded in agarose on a microscope slide, lysed and subjected to electrophoresis causing DNA and its fragments to migrate in the gel. The patterns ("comets") formed by the migration indicated the status of DNA. The comets were evaluated both with a fluorescence microscope by visual inspection (H.C.) and computer-assisted image analysis (G.K.). On day 1, a majority of the cells showed comets with very short tails, or no tail at all. A few cells showed advanced stages of DNA degradation. The number of these cells increased with time, as well as the degree of degradation. The bacterial contamination, which was rare on day 1, increased with time, and gave a characteristic background to day 10 samples. It was concluded that the neutral comet assay could be used as a method to rapidly screen fresh chicken in order to assess its quality.
European Food Research and Technology – Springer Journals
Published: Jun 24, 1998
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera