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DNA and RNA Isolation Techniques for Non-ExpertsIsolation of Bacteriophage DNA by PEG Method

DNA and RNA Isolation Techniques for Non-Experts: Isolation of Bacteriophage DNA by PEG Method [The polyethylene glycol (PEG) method is one of the most appropriate approaches for extracting DNA from bacteriophages. The standard method of preparing DNA entails numerous expensive, time-consuming processes and delivers low DNA yields. This procedure, on the other hand, is quite simple and rapid. The PEG method is based on the solubilization of phase particles and macromolecular crowding of DNA, which may then be purified with phenol and precipitated with ethanol. The concentration of PEG required for precipitation is inversely proportional to the size of the DNA fragments. This method can also be used to extract DNA from bacteria and fungi.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsIsolation of Bacteriophage DNA by PEG Method

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References (2)

  • JR Clark (2004)

    463

    Expert Review of Vaccines, 3

  • D Atha (1981)

    12108

    Journal of Biological Chemistry, 256

Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
73 –78
DOI
10.1007/978-3-030-94230-4_9
Publisher site
See Chapter on Publisher Site

Abstract

[The polyethylene glycol (PEG) method is one of the most appropriate approaches for extracting DNA from bacteriophages. The standard method of preparing DNA entails numerous expensive, time-consuming processes and delivers low DNA yields. This procedure, on the other hand, is quite simple and rapid. The PEG method is based on the solubilization of phase particles and macromolecular crowding of DNA, which may then be purified with phenol and precipitated with ethanol. The concentration of PEG required for precipitation is inversely proportional to the size of the DNA fragments. This method can also be used to extract DNA from bacteria and fungi.]

Published: Mar 30, 2022

Keywords: Polyethylene glycol; Genetic engineering; Vaccine; Bacteriophage; E. coli

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