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DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Hydrophilic Ionic Liquid Treatment

DNA and RNA Isolation Techniques for Non-Experts: DNA Isolation by Hydrophilic Ionic Liquid... [For quick and quantitative DNA extraction, hydrophilic ionic liquids (ILs) are highly beneficial. This DNA extraction procedure is affordable and takes only a few minutes to complete. Ionic liquids can dissolve biomass and release DNA molecules from tissues and cells. DNA released in the supernatant may be used as such for downstream applications. The extraction procedure includes mixing ionic liquids with starting sample at a high temperature (65–95 °C), depending upon the sample source. If the plant or animal tissues are being used, brief centrifugation may be needed. Aqueous supernatant may be directly used for downstream applications. This approach is suited for high sample throughput and allows DNA extraction from bacteria, plant, and animal tissues in resource-constrained environments. Therefore, this method is most suitable for the detection of contaminations in food. The major disadvantage of this approach is that it might result in highly diluted DNA, which requires subsequent concentration by using DNA-binding columns or silica-coated magnetic beads.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Hydrophilic Ionic Liquid Treatment

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
63 –68
DOI
10.1007/978-3-030-94230-4_7
Publisher site
See Chapter on Publisher Site

Abstract

[For quick and quantitative DNA extraction, hydrophilic ionic liquids (ILs) are highly beneficial. This DNA extraction procedure is affordable and takes only a few minutes to complete. Ionic liquids can dissolve biomass and release DNA molecules from tissues and cells. DNA released in the supernatant may be used as such for downstream applications. The extraction procedure includes mixing ionic liquids with starting sample at a high temperature (65–95 °C), depending upon the sample source. If the plant or animal tissues are being used, brief centrifugation may be needed. Aqueous supernatant may be directly used for downstream applications. This approach is suited for high sample throughput and allows DNA extraction from bacteria, plant, and animal tissues in resource-constrained environments. Therefore, this method is most suitable for the detection of contaminations in food. The major disadvantage of this approach is that it might result in highly diluted DNA, which requires subsequent concentration by using DNA-binding columns or silica-coated magnetic beads.]

Published: Mar 30, 2022

Keywords: Ionic liquids; qPCR; Choline hexanoate; PCR enhancement; Food safety

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