Diverse roles of PtrDUF579 proteins in Populus and PtrDUF579-1 function in vascular cambium proliferation during secondary growth

Diverse roles of PtrDUF579 proteins in Populus and PtrDUF579-1 function in vascular cambium... DUF579 (domain of unknown function 579) family proteins contain a DUF579 domain structure but vary greatly in their overall sequence similarity. Several DUF579 proteins have been found to play a role in cell wall biosynthesis in Arabidopsis, while DUF579 family genes have not yet been systematically investigated in Populus. In this study, the Populus DUF579 family proteins were found to be localized in different cell types and subcellular locations. The diverse expression patterns of the proteins indicate that they may perform different functions in Populus. Among the DUF579 family members, PtrDUF579-1 is found to be specifically expressed in vascular cambium zone cells where it is localized in the Golgi apparatus. Suppression of PtrDUF579-1 expression reduced plant height and stem diameter size. Cambium cell division and xylem tissue growth was inhibited while secondary cell wall formation was unchanged in PtrDUF579-1 suppressed plants. Cell walls analysis showed that the composition of the pectin fraction of the cambium cell wall was altered while other polysaccharides were not affected in PtrDUF579-1 suppressed plants. This observation suggest cambium expressed PtrDUF579-1 may affect cell wall biosynthesis and be involved in cambium cell proliferation in Populus. Overall, DUF579 family proteins play a diverse set of roles in Populus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Diverse roles of PtrDUF579 proteins in Populus and PtrDUF579-1 function in vascular cambium proliferation during secondary growth

12 pages
Read Article

Loading next page...
 
/lp/springer-journals/diverse-roles-of-ptrduf579-proteins-in-populus-and-ptrduf579-1-U9uEwgCEEq
Publisher
Springer Journals
Copyright
Copyright © 2014 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-014-0206-9
Publisher site
See Article on Publisher Site

Abstract

DUF579 (domain of unknown function 579) family proteins contain a DUF579 domain structure but vary greatly in their overall sequence similarity. Several DUF579 proteins have been found to play a role in cell wall biosynthesis in Arabidopsis, while DUF579 family genes have not yet been systematically investigated in Populus. In this study, the Populus DUF579 family proteins were found to be localized in different cell types and subcellular locations. The diverse expression patterns of the proteins indicate that they may perform different functions in Populus. Among the DUF579 family members, PtrDUF579-1 is found to be specifically expressed in vascular cambium zone cells where it is localized in the Golgi apparatus. Suppression of PtrDUF579-1 expression reduced plant height and stem diameter size. Cambium cell division and xylem tissue growth was inhibited while secondary cell wall formation was unchanged in PtrDUF579-1 suppressed plants. Cell walls analysis showed that the composition of the pectin fraction of the cambium cell wall was altered while other polysaccharides were not affected in PtrDUF579-1 suppressed plants. This observation suggest cambium expressed PtrDUF579-1 may affect cell wall biosynthesis and be involved in cambium cell proliferation in Populus. Overall, DUF579 family proteins play a diverse set of roles in Populus.

Journal

Plant Molecular Biology – Springer Journals

Published: Jun 5, 2014

References

  • Isolation and structural characterization of β-d-glucosyluronic acid and 4-O-methyl β-D-glucosyluronic acid-containing oligosaccharides from the cell-wall pectic polysaccharide, rhamnogalacturonan I
    An, J; O’Neill, MA; Albersheim, P; Darvill, AG
  • Evolving views of pectin synthesis
    Atmodjo, MA; Hao, Z; Mohnen, D
  • QUASIMODO1 encodes a putative membrane-bound glycosyltransferase required for normal pectin synthesis and cell adhesion in Arabidopsis
    Bouton, S; Leboeuf, E; Mouille, G; Leydecker, MT; Talbotec, J; Granier, F; Lahaye, M; Hofte, H; Truong, HN
    Bookmark
  • Arabidopsis genes IRREGULAR XYLEM (IRX15) and IRX15L encode DUF579-containing proteins that are essential for normal xylan deposition in the secondary cell wall
    Brown, D; Wightman, R; Zhang, Z; Gomez, LD; Atanassov, I; Bukowski, JP; Tryfona, T; McQueen-Mason, SJ; Dupree, P; Turner, S
  • The structure, function, and biosynthesis of plant cell wall pectic polysaccharides
    Caffall, KH; Mohnen, D
    Bookmark
  • Pectic arabinan side chains are essential for pollen cell wall integrity during pollen development
    Cankar, K; Kortstee, A; Toonen, MA; Wolters-Arts, M; Houbein, R; Mariani, C; Ulvskov, P; Jorgensen, B; Schols, HA; Visser, RG
    Bookmark
  • A simple and efficient method for isolating RNA from pine trees
    Chang, S; Puryear, J; Cairney, J
  • Plant Cell Walls
    Gaspar, Y; Johnson, KL; McKenna, JA; Bacic, A; Schultz, CJ
  • Biosynthesis of pectin
    Harholt, J; Suttangkakul, A; Vibe Scheller, H
  • A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem
    Iwai, H; Masaoka, N; Ishii, T; Satoh, S
  • The DUF579 domain containing proteins IRX15 and IRX15-L affect xylan synthesis in Arabidopsis
    Jensen, JK; Kim, H; Cocuron, JC; Orler, R; Ralph, J; Wilkerson, CG
  • Shotgun proteome profile of Populus developing xylem
    Kalluri, UC; Hurst, GB; Lankford, PK; Ranjan, P; Pelletier, DA
  • Three Arabidopsis DUF579 domain-containing GXM proteins are methyltransferases catalyzing 4-O-methylation of glucuronic acid on xylan
    Lee, C; Teng, Q; Zhong, R; Yuan, Y; Haghighat, M; Ye, ZH
  • Combinatorial modification of multiple lignin traits in trees through multigene cotransformation
    Li, L; Zhou, Y; Cheng, X; Sun, J; Marita, JM; Ralph, J; Chiang, VL
  • Rice cellulose synthase-like D4 is essential for normal cell-wall biosynthesis and plant growth
    Li, M; Xiong, G; Li, R; Cui, J; Tang, D; Zhang, B; Pauly, M; Cheng, Z; Zhou, Y
  • Analysis of plant gums and saccharide materials in paint samples: comparison of GC–MS analytical procedures and databases
    Lluveras-Tenorio, A; Mazurek, J; Restivo, A; Colombini, MP; Bonaduce, I
  • Pectin structure and biosynthesis
    Mohnen, D
  • A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants
    Nelson, BK; Cai, X; Nebenfuhr, A
  • Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth
    O’Neill, MA; Eberhard, S; Albersheim, P; Darvill, AG
  • Characterization of cellulose synthase complexes in Populus xylem differentiation
    Song, D; Shen, J; Li, L
  • Characterization of the plasma membrane proteins and receptor-like kinases associated with secondary vascular differentiation in poplar
    Song, D; Xi, W; Shen, J; Bi, T; Li, L
  • The plant Golgi apparatus: structure, functional organization and trafficking mechanisms
    Staehelin, LA; Moore, I
  • MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
    Tamura, K; Peterson, D; Peterson, N; Stecher, G; Nei, M; Kumar, S
  • Chemistry and uses of pectin—a review
    Thakur, BR; Singh, RK; Handa, AK; Rao, M
  • Structural characterization of Arabidopsis leaf arabinogalactan polysaccharides
    Tryfona, T; Liang, H-C; Kotake, T; Tsumuraya, Y; Stephens, E; Dupree, P
  • The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
    Tuskan, GA; Difazio, S; Jansson, S; Bohlmann, J; Grigoriev, I; Hellsten, U; Putnam, N; Ralph, S; Rombauts, S; Salamov, A
  • 4-O-methylation of glucuronic acid in Arabidopsis glucuronoxylan is catalyzed by a domain of unknown function family 579 protein
    Urbanowicz, BR; Peña, MJ; Ratnaparkhe, S; Avci, U; Backe, J; Steet, HF; Foston, M; Li, H; O’Neill, MA; Ragauskas, AJ
  • Identification and biochemical characterization of four wood-associated glucuronoxylan methyltransferases in Populus
    Yuan, Y; Teng, Q; Zhong, R; Ye, Z-H

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial