Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Differential medium for revelation of bacterial producer strains of L-asparaginases

Differential medium for revelation of bacterial producer strains of L-asparaginases A specific, fast, and easy method for revelation of active plate producers of L-asparaginase using differential medium on the basis of LB or M9 with 1.5% agar was developed. Each 100 ml of LB or M9 medium additionally contained 6–7 ml of glycerol, 4 g of L-asparagine, 0.2 g of CaCO3, and diagnostic components: 3 ml of 0.2 M CuSO4 · 5H2O and 2.5 ml of 0.1 M K3Fe(CN)6, pH 7.6–7.8. The results were counted 12–20 or 24–48 h after strain growth at 37°C in corresponding mediums. Red color of colonies and colored zone around them showed the ability of the strain under study to destroy asparaginic complexes. The recommended method allows revealing bacterial strains producing L-asparaginase with specific activity of not less than 0.1–3.0 MU/mg of protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Biochemistry and Microbiology Springer Journals

Differential medium for revelation of bacterial producer strains of L-asparaginases

Download PDF
4 pages

Loading next page...
 
/lp/springer-journals/differential-medium-for-revelation-of-bacterial-producer-strains-of-l-eQIuK4bl3N

References (11)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Medical Microbiology ; Microbiology ; Biochemistry, general
ISSN
0003-6838
eISSN
1608-3024
DOI
10.1134/S0003683811020165
Publisher site
See Article on Publisher Site

Abstract

A specific, fast, and easy method for revelation of active plate producers of L-asparaginase using differential medium on the basis of LB or M9 with 1.5% agar was developed. Each 100 ml of LB or M9 medium additionally contained 6–7 ml of glycerol, 4 g of L-asparagine, 0.2 g of CaCO3, and diagnostic components: 3 ml of 0.2 M CuSO4 · 5H2O and 2.5 ml of 0.1 M K3Fe(CN)6, pH 7.6–7.8. The results were counted 12–20 or 24–48 h after strain growth at 37°C in corresponding mediums. Red color of colonies and colored zone around them showed the ability of the strain under study to destroy asparaginic complexes. The recommended method allows revealing bacterial strains producing L-asparaginase with specific activity of not less than 0.1–3.0 MU/mg of protein.

Journal

Applied Biochemistry and Microbiology – Springer Journals

Published: Mar 9, 2011

There are no references for this article.