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Development of a transgenic hairy root system in jute ( Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation

Development of a transgenic hairy root system in jute ( Corchorus capsularis L.) with gusA... Transgenic hairy root system is important in several recalcitrant plants, where Agrobacterium tumefaciens -mediated plant transformation and generation of transgenic plants are problematic. Jute ( Corchorus spp.), the major fibre crop in Indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, Agrobacterium -mediated genetic transformation remains to be a challenging proposition in this crop. In the present work, a system of transgenic hairy roots in Corchorus capsularis L. has been developed through genetic transformation by Agrobacterium rhizogenes harbouring two plasmids, i.e. the natural Ri plasmid and a recombinant binary vector derived from the disarmed Ti plasmid of A. tumefaciens . Our findings indicate that the system is relatively easy to establish and reproducible. Molecular analysis of the independent lines of transgenic hairy roots revealed the transfer of relevant transgenes from both the T-DNA parts into the plant genome, indicating the co-transformation nature of the event. High level expression and activity of the gusA reporter gene advocate that the transgenic hairy root system, thus developed, could be applicable as gene expression system in general and for root functional genomics in particular. Furthermore, these transgenic hairy roots can be used in future as explants for plantlet regeneration to obtain stable transgenic jute plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Development of a transgenic hairy root system in jute ( Corchorus capsularis L.) with gusA reporter gene through Agrobacterium rhizogenes mediated co-transformation

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Life Sciences; Cell Biology; Plant Biochemistry; Plant Sciences ; Biotechnology
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-010-0957-y
pmid
21153028
Publisher site
See Article on Publisher Site

Abstract

Transgenic hairy root system is important in several recalcitrant plants, where Agrobacterium tumefaciens -mediated plant transformation and generation of transgenic plants are problematic. Jute ( Corchorus spp.), the major fibre crop in Indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, Agrobacterium -mediated genetic transformation remains to be a challenging proposition in this crop. In the present work, a system of transgenic hairy roots in Corchorus capsularis L. has been developed through genetic transformation by Agrobacterium rhizogenes harbouring two plasmids, i.e. the natural Ri plasmid and a recombinant binary vector derived from the disarmed Ti plasmid of A. tumefaciens . Our findings indicate that the system is relatively easy to establish and reproducible. Molecular analysis of the independent lines of transgenic hairy roots revealed the transfer of relevant transgenes from both the T-DNA parts into the plant genome, indicating the co-transformation nature of the event. High level expression and activity of the gusA reporter gene advocate that the transgenic hairy root system, thus developed, could be applicable as gene expression system in general and for root functional genomics in particular. Furthermore, these transgenic hairy roots can be used in future as explants for plantlet regeneration to obtain stable transgenic jute plants.

Journal

Plant Cell ReportsSpringer Journals

Published: Apr 1, 2011

References