Development of a method for genetic identification of four species of anchovies: E. encrasicolus, E. anchoita, E. ringens and E. japonicus

Development of a method for genetic identification of four species of anchovies: E. encrasicolus,... Cytochrome b has been successfully employed for genetic identification of four species of anchovies (Engraulis spp) using two methodologies: PCR–RFLP and FINS. The first method allowed the identification of Engraulis anchoita, Engraulis ringens and Engraulis japonicus–Engraulis encrasicolus. In some cases, with a determined restriction profile, this technique was able to differentiate E. japonicus from E. encrasicolus. The second method allowed the identification of those four species and demonstrates that FINS is a suitable technique for the identification of all species studied in this work. Phylogenetic trees show that sequences of E. encrasicolus are grouped into two different clusters. These results are consistent with the previously published data which suggest that some species of genus Engraulis could be cryptic species, being one specie or population distributed in the oceanic habitat and the other one around the coast. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Food Research and Technology Springer Journals

Development of a method for genetic identification of four species of anchovies: E. encrasicolus, E. anchoita, E. ringens and E. japonicus

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Publisher
Springer Journals
Copyright
Copyright © 2006 by Springer-Verlag
Subject
Chemistry; Forestry; Agriculture; Biotechnology; Analytical Chemistry ; Food Science
ISSN
1438-2377
eISSN
1438-2385
D.O.I.
10.1007/s00217-005-0241-5
Publisher site
See Article on Publisher Site

Abstract

Cytochrome b has been successfully employed for genetic identification of four species of anchovies (Engraulis spp) using two methodologies: PCR–RFLP and FINS. The first method allowed the identification of Engraulis anchoita, Engraulis ringens and Engraulis japonicus–Engraulis encrasicolus. In some cases, with a determined restriction profile, this technique was able to differentiate E. japonicus from E. encrasicolus. The second method allowed the identification of those four species and demonstrates that FINS is a suitable technique for the identification of all species studied in this work. Phylogenetic trees show that sequences of E. encrasicolus are grouped into two different clusters. These results are consistent with the previously published data which suggest that some species of genus Engraulis could be cryptic species, being one specie or population distributed in the oceanic habitat and the other one around the coast.

Journal

European Food Research and TechnologySpringer Journals

Published: Feb 3, 2006

References

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