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Development of a highly efficient, repetitive system of organogenesis in soybean ( Glycine max (L.) Merr).

Development of a highly efficient, repetitive system of organogenesis in soybean ( Glycine max... A highly efficient, repetitive system of organogenesis was developed in soybean. Seeds of soybean cv. ‘White hilum’ pretreated with TDZ formed multiple bud tissue(s) (MBT) at the cotyledonary nodes. MBT initiation occurred only if the axillary buds were not removed from the cotyledonary node. The best MBT formation was achieved by pretreating the seeds for 1 week on medium supplemented with 0.1 mg/l TDZ, followed by culture of the cotyledonary node on medium supplemented with 0.5 mg/l BA for 4 weeks. Culture of the MBT on medium supplemented with 0.1 mg/l TDZ resulted in the proliferation of MBT. MBT was maintained in this way for 12 months. Three hundred thirty six shoots were obtained when 1 g of MBT was subcultured on medium supplemented with 0.5 mg/l BA. Plants were rooted on medium without growth regulators. The regenerated plants grew normally in the greenhouse. Unfortunately, they did not set seeds because of the long-day conditions during growth. This system was successfully applied in three other genotypes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Development of a highly efficient, repetitive system of organogenesis in soybean ( Glycine max (L.) Merr).

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References (32)

Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer-Verlag
Subject
LifeSciences
ISSN
0721-7714
eISSN
1432-203X
DOI
10.1007/s00299-005-0971-7
pmid
16075225
Publisher site
See Article on Publisher Site

Abstract

A highly efficient, repetitive system of organogenesis was developed in soybean. Seeds of soybean cv. ‘White hilum’ pretreated with TDZ formed multiple bud tissue(s) (MBT) at the cotyledonary nodes. MBT initiation occurred only if the axillary buds were not removed from the cotyledonary node. The best MBT formation was achieved by pretreating the seeds for 1 week on medium supplemented with 0.1 mg/l TDZ, followed by culture of the cotyledonary node on medium supplemented with 0.5 mg/l BA for 4 weeks. Culture of the MBT on medium supplemented with 0.1 mg/l TDZ resulted in the proliferation of MBT. MBT was maintained in this way for 12 months. Three hundred thirty six shoots were obtained when 1 g of MBT was subcultured on medium supplemented with 0.5 mg/l BA. Plants were rooted on medium without growth regulators. The regenerated plants grew normally in the greenhouse. Unfortunately, they did not set seeds because of the long-day conditions during growth. This system was successfully applied in three other genotypes.

Journal

Plant Cell Reports – Springer Journals

Published: Nov 1, 2005

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