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- Publisher
- Springer Journals
- Copyright
- Copyright © 2009 by Vieweg+Teubner | GWV Fachverlage GmbH
- Subject
- Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine
- ISSN
- 0009-5893
- eISSN
- 1612-1112
- DOI
- 10.1365/s10337-009-1162-4
- Publisher site
- See Article on Publisher Site
Abstract
A highly sensitive and selective method for determination of histamine in rat plasma and tissue extracts by liquid chromatography with fluorescence detection is described. The method is based on precolumn derivatization of amino groups of histamine with two molecules of 4-(1-pyrene)butyric acid N-hydroxysuccinimide ester that allows generation of intramolecular excimer fluorescence. The detection limit for histamine was 0.183 nM. This sensitivity allowed determination of histamine in 10 μL of rat plasma or in the extracts from less than 1 mg of tissue.
Journal
Chromatographia – Springer Journals
Published: Jun 16, 2009