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Cytology of bone marrow in acute, sub-acute and chronic CCL4-toxicity in female Wistar rats

Cytology of bone marrow in acute, sub-acute and chronic CCL4-toxicity in female Wistar rats The cytopathology of carbon tetrachloride (CCl4) in the liver and kidney has been widely studied and well documented. This study is a look at bone marrow responses to acute, sub-acute and chronic CCl4 toxicities in female Wistar rats. Sixteen (16) female Wistar rats, with a mean body weight of 180 g, were used for this study. The rats were randomly allocated into four groups of four rats each. Group I served as the control and was untreated. Group II was the acute study group and received 20% CCl4 at 3 mL/kg body weight, intraperitoneally (i.p.), stat. Group III was the sub-acute study group and received 15% CCl4 at 3 mL/kg body weight, i.p. every 5 days for 28 days, while group IV rats were the chronic study group and received 10% CCl4 at 3 mL/kg body weight, i.p., every 5 days for 90 days. The proliferation and maturation of both the erythroid and myeloid cell lines were evaluated using indices that analysed the rates and ratios of proliferating blast cells to mature cells. Results indicated that the myeloid to erythroid ratio was not significantly affected by CCL4 toxicity, and proliferation indices showed a significant increase only at the sub-acute level of the myeloid series. However sub-acute and chronic CCl4 toxicity significantly affected metamyelocytes maturation. Thus, this study showed that while the bone marrow still retained haematopoietic responsiveness under CCl4 toxicity, at sub-acute and chronic levels, the latter stages of myelocyte maturation were adversely affected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Comparative Clinical Pathology Springer Journals

Cytology of bone marrow in acute, sub-acute and chronic CCL4-toxicity in female Wistar rats

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Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2022
eISSN
1618-565X
DOI
10.1007/s00580-022-03378-6
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Abstract

The cytopathology of carbon tetrachloride (CCl4) in the liver and kidney has been widely studied and well documented. This study is a look at bone marrow responses to acute, sub-acute and chronic CCl4 toxicities in female Wistar rats. Sixteen (16) female Wistar rats, with a mean body weight of 180 g, were used for this study. The rats were randomly allocated into four groups of four rats each. Group I served as the control and was untreated. Group II was the acute study group and received 20% CCl4 at 3 mL/kg body weight, intraperitoneally (i.p.), stat. Group III was the sub-acute study group and received 15% CCl4 at 3 mL/kg body weight, i.p. every 5 days for 28 days, while group IV rats were the chronic study group and received 10% CCl4 at 3 mL/kg body weight, i.p., every 5 days for 90 days. The proliferation and maturation of both the erythroid and myeloid cell lines were evaluated using indices that analysed the rates and ratios of proliferating blast cells to mature cells. Results indicated that the myeloid to erythroid ratio was not significantly affected by CCL4 toxicity, and proliferation indices showed a significant increase only at the sub-acute level of the myeloid series. However sub-acute and chronic CCl4 toxicity significantly affected metamyelocytes maturation. Thus, this study showed that while the bone marrow still retained haematopoietic responsiveness under CCl4 toxicity, at sub-acute and chronic levels, the latter stages of myelocyte maturation were adversely affected.

Journal

Comparative Clinical Pathology – Springer Journals

Published: Oct 1, 2022

Keywords: Carbon tetrachloride; Toxicity; Bone marrow; Cytology

References

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