Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Nunes, Raquel Cristóvão, M. Freire, V. Santos-Ebinuma, J. Faria, Cláudia Silva, Ana Tavares (2020)
Recent Strategies and Applications for l-Asparaginase ConfinementMolecules, 25
M. Dumina, M. Eldarov, D. Zdanov, N. Sokolov (2020)
L-Asparaginases of Extremophilic Microorganisms in BiomedicineBiochemistry (Moscow), Supplement Series B: Biomedical Chemistry, 14
Shaohua Zuo, Tao Zhang, B. Jiang, W. Mu (2015)
Reduction of acrylamide level through blanching with treatment by an extremely thermostable l-asparaginase during French fries processingExtremophiles, 19
Shaohua Zuo, D. Xue, Tao Zhang, B. Jiang, W. Mu (2014)
Biochemical characterization of an extremely thermostable l-asparaginase from Thermococcus gammatolerans EJ3Journal of Molecular Catalysis B-enzymatic, 109
M. Duval, S. Suciu, A. Ferster, X. Rialland, B. Nelken, P. Lutz, Y. Benoît, A. Robert, A. Manel, E. Vilmer, J. Otten, N. Philippe (2002)
Comparison of Escherichia coli-asparaginase with Erwinia-asparaginase in the treatment of childhood lymphoid malignancies: results of a randomized European Organisation for Research and Treatment of Cancer-Children's Leukemia Group phase 3 trial.Blood, 99 8
A. Mardanov, Vitali Svetlitchnyi, A. Beletsky, M. Prokofeva, E. Bonch‐Osmolovskaya, N. Ravin, K. Skryabin (2010)
The Genome Sequence of the Crenarchaeon Acidilobus saccharovorans Supports a New Order, Acidilobales, and Suggests an Important Ecological Role in Terrestrial Acidic Hot SpringsApplied and Environmental Microbiology, 76
A. Farag, S. Hassan, E. Beltagy, M. El-Shenawy (2015)
Optimization of production of anti-tumor l-asparaginase by free and immobilized marine Aspergillus terreusThe Egyptian Journal of Aquatic Research, 41
(2017)
Acta Crystallogr
Noura El-Nagga, Sara El-Ewasy, Nancy El-Shweihy (2014)
Microbial L-asparaginase as a Potential Therapeutic Agent for the Treatment of Acute Lymphoblastic Leukemia: The Pros and ConsInternational Journal of Pharmacology, 10
M.V. Dumina (2020)
10.18097/pbmc20206602105Biomed. Khim, 66
Shahid Chohan, N. Rashid (2013)
TK1656, a thermostable l-asparaginase from Thermococcus kodakaraensis, exhibiting highest ever reported enzyme activity.Journal of bioscience and bioengineering, 116 4
J. Varshosaz, Negin Anvari (2017)
Enhanced stability of L-asparaginase by its bioconjugation to poly(styrene-co-maleic acid) and Ecoflex nanoparticles.IET nanobiotechnology, 12 4
Sudhir Kumar, G. Stecher, Michael Li, Christina Knyaz, K. Tamura (2018)
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.Molecular biology and evolution, 35 6
Anup Ashok, K. Doriya, J. Rao, Asif Qureshi, A. Tiwari, D. Kumar (2019)
Microbes Producing L-Asparaginase free of Glutaminase and Urease isolated from Extreme Locations of Antarctic Soil and MossScientific Reports, 9
A.V. Mardanov, A.V. Beletsky, V.V. Kadnikov, A.N. Ignatov, N.V. Ravin (2014)
24459262Genome Announc, 2
A. Safary, R. Moniri, Maryam Hamzeh-Mivehroud, S. Dastmalchi (2018)
Highly efficient novel recombinant L-asparaginase with no glutaminase activity from a new halo-thermotolerant Bacillus strainBioImpacts : BI, 9
T. Hoshino, Nan Xiao, O. Tkachenko (2009)
Cold adaptation in the phytopathogenic fungi causing snow moldsMycoscience, 50
O. Podosokorskaya, V. Kadnikov, S. Gavrilov, A. Mardanov, A. Merkel, O. Karnachuk, N. Ravin, E. Bonch‐Osmolovskaya, I. Kublanov (2013)
Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae.Environmental microbiology, 15 6
S. Warangkar, C. Khobragade (2009)
Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-AsparaginaseEnzyme Research, 2010
A. Pritsa, D. Kyriakidis (2004)
L-asparaginase of Thermus thermophilus: Purification, properties and identificaation of essential amino acids for its catalytic activityMolecular and Cellular Biochemistry, 216
S. Altschul, Thomas Madden, A. Schäffer, Jinghui Zhang, Zheng Zhang, W. Miller, D. Lipman (1997)
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic acids research, 25 17
Monique Curran, Roy Daniel, Graeme Guy, Hugh Morgan (1985)
A specific L-asparaginase from Thermus aquaticus.Archives of biochemistry and biophysics, 241 2
N. Sokolov, M. Eldarov, M. Pokrovskaya, S. Aleksandrova, O. Abakumova, O. Podobed, N. Melik-Nubarov, Elena Kudryashova, D. Grishin, A. Archakov (2015)
[Bacterial recombinant L-asparaginases: properties, structure and anti-proliferative activity].Biomeditsinskaia khimiia, 61 3
T. Hoshino, F. Terami, O. Tkachenko, M. Tojo, N. Matsumoto (2010)
Mycelial growth of the snow mold fungus, Sclerotinia borealis, improved at low water potentials: an adaption to frozen environmentMycoscience, 51
Ju Li, Jianquan Wang, L. Bachas (2002)
Biosensor for asparagine using a thermostable recombinant asparaginase from Archaeoglobus fulgidus.Analytical chemistry, 74 14
A. Gunny, D. Arbain, P. Jamal, Rizo Gumba (2014)
Improvement of halophilic cellulase production from locally isolated fungal strainSaudi Journal of Biological Sciences, 22
Eira Kelo, T. Noronkoski, I. Stoineva, D. Petkov, I. Mononen (2002)
β‐Aspartylpeptides as substrates of L‐asparaginases from Escherichia coli and Erwinia chrysanthemiFEBS Letters, 528
Shahid Chohan, N. Rashid, Muhammad Sajed, T. Imanaka (2018)
Pcal_0970: an extremely thermostable l-asparaginase from Pyrobaculum calidifontis with no detectable glutaminase activityFolia Microbiologica, 64
G. Baskar, R. Aiswarya (2018)
Overview on mitigation of acrylamide in starchy fried and baked foods.Journal of the science of food and agriculture, 98 12
A. Zhgun, G. Nuraeva, M. Dumina, T. Voinova, V. Dzhavakhiya, M. Eldarov (2019)
1,3-Diaminopropane and Spermidine Upregulate Lovastatin Production and Expression of Lovastatin Biosynthetic Genes in Aspergillus terreus via LaeA RegulationApplied Biochemistry and Microbiology, 55
M. Pokrovskaya, S. Aleksandrova, V. Pokrovsky, A. Veselovsky, D. Grishin, O. Abakumova, O. Podobed, A. Mishin, D. Zhdanov, N. Sokolov (2015)
Identification of Functional Regions in the Rhodospirillum rubruml-Asparaginase by Site-Directed MutagenesisMolecular Biotechnology, 57
H. Wade, H. Robinson, B. Phillips (1971)
Asparaginase and glutaminase activities of bacteria.Journal of general microbiology, 69 3
L. Azeredo, D. Freire, R. Soares, S. Leite, R. Coelho (2004)
Production and partial characterization of thermophilic proteases from Streptomyces sp. isolated from Brazilian cerrado soilEnzyme and Microbial Technology, 34
(2014)
Basic Microbiol
I. Moguel, C. Yamakawa, A. Pessoa, S. Mussatto (2020)
L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of LipidsFrontiers in Bioengineering and Biotechnology, 8
A. Duarte, Mariana Barato, F. Nobre, D. Polezel, T. Oliveira, J. Santos, A. Rodrigues, L. Sette (2018)
Production of cold-adapted enzymes by filamentous fungi from King George Island, AntarcticaPolar Biology, 41
T. Hatanaka, H. Usuki, J. Arima, Yoshiko Uesugi, Yukihiro Yamamoto, Yuya Kumagai, A. Yamasato, T. Mukaihara (2011)
Extracellular Production and Characterization of Two Streptomycesl-AsparaginasesApplied Biochemistry and Biotechnology, 163
F. Sarmiento, R. Peralta, J. Blamey (2015)
Cold and Hot Extremozymes: Industrial Relevance and Current TrendsFrontiers in Bioengineering and Biotechnology, 3
R. Aiswarya, G. Baskar (2018)
Enzymatic mitigation of acrylamide in fried potato chips using asparaginase from Aspergillus terreusInternational Journal of Food Science & Technology, 53
B. Burg (2003)
Extremophiles as a source for novel enzymes.Current Opinion in Microbiology, 6
N. Danilova (2020)
ExtremophilesStress Response and Immunity: Links and Trade Offs
A. Zhgun, M. Dumina, T. Voinova, V. Dzhavakhiya, M. Eldarov (2018)
Role of acetyl-CoA Synthetase and LovE Regulator Protein of Polyketide Biosynthesis in Lovastatin Production by Wild-Type and Overproducing Aspergillus terreus StrainsApplied Biochemistry and Microbiology, 54
Joshua Heuslein, Stephanie McDonnell, Ji Song, B. Annex, R. Price (2018)
MicroRNA-146a Regulates Perfusion Recovery in Response to Arterial Occlusion via ArteriogenesisFrontiers in Bioengineering and Biotechnology, 6
Yosuke Matsumoto, K. Nomura, Yumiko Kanda-Akano, Y. Fujita, M. Nakao, Kyoji Ueda, S. Horiike, S. Yokota, K. Kusuzaki, T. Kitoh, A. Watanabe, M. Taniwaki (2003)
Successful Treatment with Erwinia l -Asparaginase for Recurrent Natural Killer/T Cell LymphomaLeukemia & Lymphoma, 44
The activity of novel uncharacterized extremophilic L-asparaginases from the psychrophilic fungi Sclerotiniaborealis, the thermoacidophilic crenarchea Acidilobus saccharovorans, and the thermophilic bacteria Melioribacter roseus were studied. Active enzymes were produced via the expression of the native L-asparaginase gene from M. roseus (MrA) and synthetic genes encoding fungal S.borealis (SbA) and archeal A. saccharovorans (AsA) L-asparaginases after codon optimization in Escherichia coli cells. In the study, the maximum specific activity at different temperatures and pH was observed for MrA. The activity of MrA crude extract was highest at 75°С and a pH of 9.0. Metal ions (1 mM) differed in their effects on enzyme activity. Сu2+ and Zn2+ ions completely abolished enzyme activity. Changes in the specific activity of MrA crude extract in the presence of Fe3+, Ni2+, Ca2+, and Mg2+ varied within 5–28%. Our findings show that L-asparaginase of M. roseus may be a promising object for the further study of enzymatic properties and biotechnological applications.
Applied Biochemistry and Microbiology – Springer Journals
Published: Sep 1, 2021
Keywords: L-asparaginase; extremophilic microorganism; heterologous expression; anticancer drug
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.