Characterization of the highly abundant polymorphic GC-rich-repetitive sequence (PGRS) present in Mycobacterium tuberculosis

Characterization of the highly abundant polymorphic GC-rich-repetitive sequence (PGRS) present in... 203 163 163 2 2 Sylvie Poulet Stewart T. Cole +33-1-4588446 +33-1-45688953 stcole@pasteur.fr Unité de Génétique Moléculaire Bactérienne Institut Pasteur 28 rue du Docteur Roux F-75724 Paris Cedex 15 France Abstract The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was the triplet CGG, or CRR (where R is a purine), it seems likely that PGRS arose by means of triplet expansion, accounting for its polymorphism. Several copies of PGRS were linked to a conserved open reading frame. PGRS was used as the target sequence for the polymerase chain reaction in an attempt to develop a new typing technique. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Characterization of the highly abundant polymorphic GC-rich-repetitive sequence (PGRS) present in Mycobacterium tuberculosis

Archives of Microbiology, Volume 163 (2) – Feb 1, 1995

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Publisher
Springer Journals
Copyright
Copyright © 1995 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00381781
Publisher site
See Article on Publisher Site

Abstract

203 163 163 2 2 Sylvie Poulet Stewart T. Cole +33-1-4588446 +33-1-45688953 stcole@pasteur.fr Unité de Génétique Moléculaire Bactérienne Institut Pasteur 28 rue du Docteur Roux F-75724 Paris Cedex 15 France Abstract The polymorphic GC-rich repetitive sequence (PGRS) found on the chromosome of Mycobacterium tuberculosis was characterized by means of mapping, cloning and sequencing. PGRS was present in at least 26 loci and consisted of many tandem repeats of the consensus sequence CGGCGGCAA. As the core of the consensus motif was the triplet CGG, or CRR (where R is a purine), it seems likely that PGRS arose by means of triplet expansion, accounting for its polymorphism. Several copies of PGRS were linked to a conserved open reading frame. PGRS was used as the target sequence for the polymerase chain reaction in an attempt to develop a new typing technique.

Journal

Archives of MicrobiologySpringer Journals

Published: Feb 1, 1995

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