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C. Stewart, M. Adang, J. All, P. Raymer, S. Ramachandran, Wayne Parrott (1996)
Insect Control and Dosage Effects in Transgenic Canola Containing a Synthetic Bacillus thuringiensis cryIAc Gene, 112
C. Stewart (2004)
Genetically Modified Planet: Environmental Impacts of Genetically Engineered Plants
L. English, S. Brussock, T. Malvar, J. Bryson, C. Kulesza, F. Walters, S. Slatin, M. Tersch, C. Romano (1998)
Insect-resistant transgenic plantsTrends in Biotechnology, 16
B. Tabashnik, Naomi Finson, Marshall Johnson, W. Moar (1993)
Resistance to Toxins from Bacillus thuringiensis subsp. kurstaki Causes Minimal Cross-Resistance to B. thuringiensis subsp. aizawai in the Diamondback Moth (Lepidoptera: Plutellidae)Applied and Environmental Microbiology, 59
SB Narashimhulu, VL Chopra (1988)
Species specific shoot regeneration response of cotyledons of BrassicasPlant Cell Rep, 7
F.-L. Zhang, Yoshihito Takahata, J.-B. Xu (1998)
Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis)Plant Cell Reports, 17
(1997)
Moran-Palma P (1997) Commercial cultivation
M. Halfhill, B. Zhu, S. Warwick, P. Raymer, Reginald Millwood, A. Weissinger, C. Stewart (2004)
Hybridization and backcrossing between transgenic oilseed rape and two related weed species under field conditions.Environmental biosafety research, 3 2
MD Halfhill, RJ Millwood, AK Weissinger, SI Warwick, CN Stewart (2003a)
Additive transgene expression and genetic introgression in multiple GFP transgenic crop x weed hybrid generationsTheor Appl Genet, 107
P. Mason, L. Braun, S. Warwick, B. Zhu, C. Neal, Stewart Jr (2003)
Transgenic Bt-producing Brassica napus: Plutella xylostella selection pressure and fitness of weedy relatives.Environmental biosafety research, 2 4
Reginald Millwood, M. Halfhill, D. Harkins, R. Russotti, C. Stewart (2003)
Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs.BioTechniques, 34 3
C. Stewart, M. Halfhill, S. Warwick (2003)
Genetic modification: Transgene introgression from genetically modified crops to their wild relativesNature Reviews Genetics, 4
R. Roush (1994)
Managing pests and their resistance to Bacillus thuringiensis: Can transgenic crops be better than sprays?Biocontrol Science and Technology, 4
AA Snow, P Moran-Palma (1997)
Commercial cultivation of transgene crops: potential ecological risksBioscience, 47
S. Ramachandran, G. Buntin, J. All, P. Raymer, C. Stewart (1998)
Greenhouse and field evaluations of transgenic canola against diamondback moth, Plutella xylostella, and corn earworm, Helicoverpa zeaEntomologia Experimentalis et Applicata, 88
A. Snow, P. Moran‐Palma, L. Rieseberg, Annette Wszelaki, Gerald Seiler (1998)
Fecundity, phenology, and seed dormancy of F1 wild-crop hybrids in Sunflower (Helianthus annuus, Asteraceae).American journal of botany, 85 6
V. Kuvshinov, K. Koivu, A. Kanerva, E. Pehu (1999)
Agrobacterium tumefaciens-mediated transformation of greenhouse-grown Brassica rapa ssp. oleiferaPlant Cell Reports, 18
G. Guéritaine, M. Sester, F. Eber, A. Chèvre, H. Darmency (2002)
Fitness of backcross six of hybrids between transgenic oilseed rape (Brassica napus) and wild radish (Raphanus raphanistrum)Molecular Ecology, 11
G. Poulsen (1996)
Genetic transformation of BrassicaPlant Breeding, 115
C. Qing, L. Fan, Y. Lei, D. Bouchez, C. Tourneur, L. Yan, C. Robaglia (2000)
Transformation of Pakchoi (Brassica rapa L. ssp. chinensis) by Agrobacterium infiltrationMolecular Breeding, 6
G. Fogg (1950)
Sinapis Arvensis L.Journal of Ecology, 38
M. Halfhill, H. Richards, S. Mabon, C. Stewart (2001)
Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapaTheoretical and Applied Genetics, 103
S. Narasimhulu, V. Chopra (1988)
Species specific shoot regeneration response of cotyledonary explants of BrassicasPlant Cell Reports, 7
G. Tucker (2008)
Raphanus raphanistrum L.
T. Mikkelsen, B. Andersen, R. Jørgensen (1996)
The risk of crop transgene spreadNature, 380
Tony Wahlroos, P. Susi, Lidia Tylkina, S. Malyshenko, S. Zvereva, T. Korpela (2003)
Agrobacterium-mediated transformation and stable expression of the green fluorescent protein in Brassica rapaPlant Physiology and Biochemistry, 41
M. Murata, T. Orton (2004)
Callus initiation and regeneration capacities in Brassica speciesPlant Cell, Tissue and Organ Culture, 11
M. Halfhill, Reginald Millwood, P. Raymer, C. Stewart (2002)
Bt-transgenic oilseed rape hybridization with its weedy relative, Brassica rapa.Environmental biosafety research, 1 1
B. Zhu, J. Lawrence, S. Warwick, P. Mason, L. Braun, M. Halfhill, C. Stewart (2004)
Stable Bacillus thuringiensis (Bt) toxin content in interspecific F1 and backcross populations of wild Brassica rapa after Bt gene transferMolecular Ecology, 13
M. Halfhill, M. Halfhill, Reginald Millwood, A. Weissinger, S. Warwick, C. Stewart (2003)
Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop × weed hybrid generationsTheoretical and Applied Genetics, 107
T. Hauser, R. Jørgensen, H. Ostergård (1998)
Fitness of backcross and F2 hybrids between weedy Brassica rapa and oilseed rape (B. napus)Heredity, 81
S. Warwick, Marie-Josée Simard, A. Légére, H. Beckie, L. Braun, B. Zhu, P. Mason, G. Séguin-Swartz, C. Stewart (2003)
Hybridization between transgenic Brassica napus L. and its wild relatives: Brassica rapa L., Raphanus raphanistrum L., Sinapis arvensis L., and Erucastrum gallicum (Willd.) O.E. SchulzTheoretical and Applied Genetics, 107
C. Stewart, M. Adang, J. All, H. Boerma, G. Cardineau, D. Tucker, Wayne Parrott (1996)
Genetic Transformation, Recovery, and Characterization of Fertile Soybean Transgenic for a Synthetic Bacillus thuringiensis cryIAc Gene, 112
S. Dellaporta, J. Wood, J. Hicks (1983)
A plant DNA minipreparation: Version IIPlant Molecular Biology Reporter, 1
H. Höfte, H. Whiteley (1989)
Insecticidal crystal proteins of Bacillus thuringiensis.Microbiological reviews, 53 2
Hani Al‐Ahmad, S. Galili, J. Gressel (2004)
Tandem constructs to mitigate transgene persistence: tobacco as a modelMolecular Ecology, 13
A. Chèvre, F. Eber, E. Jenczewski, H. Darmency, M. Renard (2003)
Gene Flow from Oilseed Rape to Weedy SpeciesActa Agriculturae Scandinavica, Section B — Soil & Plant Science, 53
C. Stewart (2004)
Genetically Modified Planet
T. Metz, R. Roush, Juliet Tang, A. Shelton, E. Earle (1995)
Transgenic broccoli expressing aBacillus thuringiensis insecticidal crystal protein: Implications for pest resistance management strategiesMolecular Breeding, 1
C. Neal, Stewart Jr, M. Halfhill, S. Warwick (2004)
Transgene introgression from genetically modified crops to their wild relativesNature Reviews Genetics, 4
S. Hobbs, T. Warkentin, Catherine DeLong (2004)
Transgene copy number can be positively or negatively associated with transgene expressionPlant Molecular Biology, 21
A. Légére (2005)
Risks and consequences of gene flow from herbicide-resistant crops: canola (Brassica napus L) as a case study.Pest management science, 61 3
M. Halfhill, J. Sutherland, Hong Moon, G. Poppy, S. Warwick, A. Weissinger, T. Rufty, P. Raymer, C. Stewart (2005)
Growth, productivity, and competitiveness of introgressed weedy Brassica rapa hybrids selected for the presence of Bt cry1Ac and gfp transgenesMolecular Ecology, 14
V. Cardoza, C. Stewart (2003)
Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explantsPlant Cell Reports, 21
sion in multiple GFP transgenic crop x weed hybrid generations
A. Snow, P. Palma (1997)
Commercialization of Transgenic Plants: Potential Ecological RisksBioScience, 47
Brian Harper, S. Mabon, Staci Leffel, M. Halfhill, H. Richards, Kari Moyer, C. Stewart (1999)
Green fluorescent protein as a marker for expression of a second gene in transgenic plantsNature Biotechnology, 17
A. Timmons, A. Timmons, Y. Charters, J. Crawford, D. Burn, Susan Scott, Susan Scott, S. Dubbels, N. Wilson, A. Robertson, Eileen O'brien, G. Squire, M. Wilkinson, M. Wilkinson (1996)
Risks from transgenic cropsNature, 380
M. Halfhill, M. Halfhill, Reginald Millwood, T. Rufty, A. Weissinger, C. Stewart (2003)
Spatial and temporal patterns of green fluorescent protein (GFP) fluorescence during leaf canopy development in transgenic oilseed rape, Brassica napus L.Plant Cell Reports, 22
C. Vacher, A. Weis, D. Hermann, Tanya Kossler, C. Young, M. Hochberg (2004)
Impact of ecological factors on the initial invasion of Bt transgenes into wild populations of birdseed rape (Brassica rapa)Theoretical and Applied Genetics, 109
Jean Molinier, Christophe Himber, Günther Hahne (2000)
Use of green fluorescent protein for detection of transformed shoots and homozygous offspringPlant Cell Reports, 19
C. Stewart (2006)
Go with the glow: fluorescent proteins to light transgenic organisms.Trends in biotechnology, 24 4
Theor Appl Genet
CN Stewart, MJ Adang, JN All, PL Raymer, S Ramachandran, WA Parrott (1996)
Insect control and dosage effects in transgenic canola, Brassica napus L. (Brassicaceae), containing a synthetic Bacillus thuringiensis cry1A(c) genePlant Physiol, 112
Crop to weed transgene flow, which could result in more competitive weed populations, is an agricultural biosafety concern. Crop Brassica napus to weedy Brassica rapa hybridization has been extensively characterized to better understand the transgene flow and its consequences. In this study, weedy accessions of B. rapa were transformed with Bacillus thuringiensis (Bt) cry1Ac- and green fluorescence protein ( gfp )-coding transgenes using Agrobacterium to assess ecological performance of the wild biotype relative to introgressed hybrids in which the transgenic parent was the crop. Regenerated transgenic B. rapa events were characterized by progeny analysis, Bt protein enzyme-linked immunosorbent assay (ELISA), Southern blot analysis, and GFP expression assay. GFP expression level and Bt protein concentration were significantly different between independent transgenic B. rapa events. Similar reproductive productivity was observed in comparison between transgenic B. rapa events and B. rapa × B. napus introgressed hybrids in greenhouse and field experiments. In the greenhouse, Bt transgenic plants experienced significantly less herbivory damage from the diamondback moth ( Plutella xylostella ). No differences were found in the field experiment under ambient, low, herbivore pressure. Directly transformed transgenic B. rapa plants should be a helpful experimental control to better understand crop genetic load in introgressed transgenic weeds.
Plant Cell Reports – Springer Journals
Published: Jul 1, 2007
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