Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Characterization of a 4 kb Variant of the nifD Element inAnabaena sp. Strain ATCC 33047

Characterization of a 4 kb Variant of the nifD Element inAnabaena sp. Strain ATCC 33047 Heterocyst differentiation in some cyanobacteria is accompanied by a programmed DNA rearrangement within the nitrogen fixation gene nifD. The nifD element is excised from within nifD during the latter stages of heterocyst differentiation by site-specific recombination. There is considerable variation in those nifD elements examined thus far, with Nostoc sp. Strain PCC 7120 and Anabaena variabilis having 11 kb elements, and Nostoc punctiforme having a 24 kb element. Here we characterize a 4 kb nifD element in Anabaena sp. Strain ATCC 33047, and compare it with the other sequenced nifD elements. While there is considerable variation in both the size (ranging from 4 kb to 24 kb) and composition of the nifD elements examined thus far, there are regions that are conserved in all. These conserved regions include the flanking 3′ and 5′ regions, the xisA gene, and a small open reading frame known as ORF2 in Nostoc sp. Strain PCC 7120. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Microbiology Springer Journals

Characterization of a 4 kb Variant of the nifD Element inAnabaena sp. Strain ATCC 33047

Current Microbiology , Volume 50 (3) – Mar 21, 2005

Loading next page...
 
/lp/springer-journals/characterization-of-a-4-kb-variant-of-the-nif-d-element-in-anabaena-sp-0GpwuaxbL0

References (27)

Publisher
Springer Journals
Copyright
Copyright © 2005 by Springer Science+Business Media, Inc.
Subject
Life Sciences; Biotechnology; Microbiology
ISSN
0343-8651
eISSN
1432-0991
DOI
10.1007/s00284-004-4338-z
pmid
15883871
Publisher site
See Article on Publisher Site

Abstract

Heterocyst differentiation in some cyanobacteria is accompanied by a programmed DNA rearrangement within the nitrogen fixation gene nifD. The nifD element is excised from within nifD during the latter stages of heterocyst differentiation by site-specific recombination. There is considerable variation in those nifD elements examined thus far, with Nostoc sp. Strain PCC 7120 and Anabaena variabilis having 11 kb elements, and Nostoc punctiforme having a 24 kb element. Here we characterize a 4 kb nifD element in Anabaena sp. Strain ATCC 33047, and compare it with the other sequenced nifD elements. While there is considerable variation in both the size (ranging from 4 kb to 24 kb) and composition of the nifD elements examined thus far, there are regions that are conserved in all. These conserved regions include the flanking 3′ and 5′ regions, the xisA gene, and a small open reading frame known as ORF2 in Nostoc sp. Strain PCC 7120.

Journal

Current MicrobiologySpringer Journals

Published: Mar 21, 2005

There are no references for this article.