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Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones



221 89 89 2 2 A. Savarese M. Annicchiarico-Petruzzelli G. Citro G. Zupi L. G. Spagnoli A. Colantoni P. Vernole A. Stephanou R. A. Knight P. Guerrieri G. Melino Dip. Medicina Sperimentale Universita Rome Tor Vergata I-00173 Rome Italy Experimental Chemotherapy Lab, Istituto Regina Elena I-00173 Rome Italy I.S.T. Genova Italy Neuroendocrinology, Charing Cross Hospital W8 London UK Brompton Hospital SW3 London UK Summary The human glioblastoma cell line LI showed morphological features typical of its neuroectodermal origin. Cells were positive by immunofluorescence to GFAP, MHC class II, and L1 determinants. Cytogenetic analysis showed the presence of a modal chromosome number of 63, ranging from 58 to 69 chromosomes (DNA index was 1.6). Northern blot analysis demonstrated the presence of mRNA transcripts specific for transglutaminase C (type II or “tissue”), growth-hormone releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and proopiomelanocortin (POMC). The GHRH mRNA was present in two different sizes, one similar to the normal hypothalamic species of 0.75 kb, whilst the second species was a large transcript of approximately 10 kb size. Treatment with 5 μM retinoic acid or 5 mM α-difluoromethylornithine for 5 days sharply reduced the growth rate and also induced modulation of the ultrastructure and antigenic profile. This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours.



Experimental Brain ResearchSpringer Journals

Published: May 1, 1992

DOI: 10.1007/BF00228256

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