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Cellulase enzyme production during continuous culture growth of Sporotrichum (Chrysosporium) thermophile

Cellulase enzyme production during continuous culture growth of Sporotrichum (Chrysosporium)... 253 24 24 4 4 Douglas Cossar Giorgio Canevascini Institut de Biologie Végétale et de Phytochimie Université de Fribourg CH-1700 Fribourg Switzerland Summary The cellulolytic fungus Sporotrichum (Chrysosporium) thermophile produces an extracellular cellobiose dehydrogenase during batch culture on cellulose or cellobiose. In chemostat culture at pH 5.6 on cellobiose this enzyme was produced in parallel with endo-cellulase. At pH 5.0 in continuous or fed-batch culture such a pattern was not evident. At constant growth rate in a chemostat with varying pH, activity of these enzymes was found to be poorly correlated. Thus the induction of cellobiose dehydrogenase shows a dependence on pH and cellobiose concentration which is different to that for endo-cellulase. The natural inducer of these enzymes and the role of cellobiose dehydrogenase remain to be elucidated. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

Cellulase enzyme production during continuous culture growth of Sporotrichum (Chrysosporium) thermophile

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References (30)

Publisher
Springer Journals
Copyright
Copyright © 1986 by Springer-Verlag
Subject
Chemistry; Biotechnology; Microbiology; Microbial Genetics and Genomics
ISSN
0175-7598
eISSN
1432-0614
DOI
10.1007/BF00257054
Publisher site
See Article on Publisher Site

Abstract

253 24 24 4 4 Douglas Cossar Giorgio Canevascini Institut de Biologie Végétale et de Phytochimie Université de Fribourg CH-1700 Fribourg Switzerland Summary The cellulolytic fungus Sporotrichum (Chrysosporium) thermophile produces an extracellular cellobiose dehydrogenase during batch culture on cellulose or cellobiose. In chemostat culture at pH 5.6 on cellobiose this enzyme was produced in parallel with endo-cellulase. At pH 5.0 in continuous or fed-batch culture such a pattern was not evident. At constant growth rate in a chemostat with varying pH, activity of these enzymes was found to be poorly correlated. Thus the induction of cellobiose dehydrogenase shows a dependence on pH and cellobiose concentration which is different to that for endo-cellulase. The natural inducer of these enzymes and the role of cellobiose dehydrogenase remain to be elucidated.

Journal

Applied Microbiology and BiotechnologySpringer Journals

Published: Jul 1, 1986

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