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- Publisher
- Springer Journals
- Copyright
- Copyright © 2007 by Springer Science+Business Media B.V.
- Subject
- Life Sciences; Biochemistry, general; Applied Microbiology; Biotechnology; Microbiology
- ISSN
- 0141-5492
- eISSN
- 1573-6776
- DOI
- 10.1007/s10529-007-9354-1
- pmid
- 17450322
- Publisher site
- See Article on Publisher Site
Abstract
Candida rugosa lipase (EC 3.1.1.3) was used to degrade commercially-available solid poly(ester)urethane (Impranil) in an aqueous medium under different temperature, pH, enzyme and substrate concentrations. A mathematical model was developed and applied to represent the degradation kinetics of the solid polyurethane. Reaction optima were found to be pH 7 and 35°C. Diethylene glycol, a degradation byproduct, generation rate was measured to be 0.12 mg/l min and the activation energy was calculated as 9.121 kcal/gmol K. This information will be useful in developing bioreactors for practical applications to manage polyurethane wastes using lipase.
Journal
Biotechnology Letters – Springer Journals
Published: Apr 21, 2007