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Callus production from protoplasts of Cyclamen persicum

Callus production from protoplasts of Cyclamen persicum Cyclamen persicum (Mill.) cv. Sierra Rose protoplasts were isolated from adventitious shoots regenerated in vitro from petiole and leaf explants with yields of 1.3 × 106 protoplasts/g f.wt. of tissue. Protoplasts were embedded within agarose lenses bathed in modified KM8p medium supplemented with 1.0 mg l-1 NAA, 0.1 mg l-1 2,4-D and 0.5 mg l-1 BA. Cell division was observed after 4–5 days. After 6 weeks calli had grown out of the lenses which were transferred to modified MS medium for further callus growth. The most rapid callus growth was on medium containing 0.1 mg l-1 NAA and 10 mg l-1 TDZ. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell, Tissue and Organ Culture Springer Journals

Callus production from protoplasts of Cyclamen persicum

Plant Cell, Tissue and Organ Culture , Volume 55 (1) – Oct 7, 2004

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References (12)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Kluwer Academic Publishers
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
0167-6857
eISSN
1573-5044
DOI
10.1023/A:1026481014379
Publisher site
See Article on Publisher Site

Abstract

Cyclamen persicum (Mill.) cv. Sierra Rose protoplasts were isolated from adventitious shoots regenerated in vitro from petiole and leaf explants with yields of 1.3 × 106 protoplasts/g f.wt. of tissue. Protoplasts were embedded within agarose lenses bathed in modified KM8p medium supplemented with 1.0 mg l-1 NAA, 0.1 mg l-1 2,4-D and 0.5 mg l-1 BA. Cell division was observed after 4–5 days. After 6 weeks calli had grown out of the lenses which were transferred to modified MS medium for further callus growth. The most rapid callus growth was on medium containing 0.1 mg l-1 NAA and 10 mg l-1 TDZ.

Journal

Plant Cell, Tissue and Organ CultureSpringer Journals

Published: Oct 7, 2004

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