Veterinary Research Communications, 30(Suppl. 1) (2006) 179–181 DOI: 10.1007/s11259-006-0035-7 C Springer 2006 Biomolecular Approaches for the Identiﬁcation of Tuna Species A. Dalmasso, T. Civera and M.T. Bottero Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, 10095, Grugliasco, Torino, Italy Correspondence: E-mail: email@example.com Dalmasso, A., Civera, T. and Bottero, M.T., 2006. Biomolecular approaches for the identiﬁcation of tuna species. Veterinary Research Communications, 30(Suppl. 1), 179–181 Keywords: PCR, sequencing, species identiﬁcation, tuna INTRODUCTION Species identiﬁcation in seafood is extremely important both for fresh products, often commercialised as ﬁllets or half-processed, and for those products submitted to techniques which modify the characteristics related to the species. The aim of this research is the application of biomolecular assays to differentiate some species of great commercial interest, such as Thunnus thynnus, Thunnus albacares, Thunnus alalunga and Katsuwonus pelamis. The differentiation of tuna ﬁsh is particularly difﬁcult as they are phylogenetically very close, thus frustrating the attempts made using electrophoretic and biomolecular techniques. In biomolecular techniques restriction enzymes have largely been used (Ram et al., 1996; Pardo and Perez-V ´ illareal, 2004); however these cannot be employed when mixed products are considered. This paper describes the design of species-speciﬁc primers able
Veterinary Research Communications – Springer Journals
Published: Jan 1, 2006
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