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Behavior of Wild-type and Transfected S2 Cells Cultured in Two Different Media



An animal protein-free medium composed of IPL-41 containing 6 g L −1 yeastolate ultrafiltrate, 10 g L −1 glucose, 2 g L −1 lactose, 5 g L −1 glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprotein from rabies virus (RVGP) and S2AcHBsAgHy-9C expressing the surface antigen of hepatitis B virus (HBsAg), both obtained from Drosophila melanogaster S2 cells. Growth of wild-type S2 cells was also evaluated in the same medium. Cell behavior in the tested medium was compared to that verified in Sf900 II®. The results show that in shake flasks, S2AcRVGP2k and S2AcHBsAgHy-9C cells reached around 2 × 10 7 cells mL −1 in both media. In supplemented IPL-41 and Sf900 II® media, S2AcRVGP2k cells produced 367 ng RVGP mL −1 and 638 ng RVGP mL −1 , respectively, while S2AcHBsAgHy-9C cells correspondently produced 573 ng HBsAg mL −1 and 322 ng HBsAg mL −1 in the mentioned media. In stirred tanks, S2AcRVGP2k cells reached 3 × 10 7 cells mL −1 and produced up to 758 ng RVGP mL −1 . In general, glucose was consumed by cells, while lactate and ammonia were produced.



Applied Biochemistry and BiotechnologySpringer Journals

Published: Jan 1, 2011

DOI: 10.1007/s12010-010-8918-z

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