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Assessment of chlorination by human neutrophils

Assessment of chlorination by human neutrophils On phagocytosing a microorganism, the neutrophil zpolymor-phonuclear leukocyte, PMN) consumes oxygen at a sharply elevated rate1. The oxygen is used to kill the microorganism, presumably being used to produce a potent oxidizing agent or agents. Candidates for these bactericidal agents are singlet oxygen, hydroxyl radical and chlorinating agents (that is, species containing ‘active’ Cl in a formal +1 oxidation state: HOCl, Cl2, N-chloroamides, and so on)1–5. We now report a semiquantitative assay for PMN-generated active chlorine based on its trapping with 1,3,5-trimethoxybenzene (TMB). Using this assay, we have found that at least 28% of the oxygen consumed by stimulated normal human PMNs is converted to active chlorinating agents. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Assessment of chlorination by human neutrophils

Foote, Christopher S.; Goyne, Thomas E.; Lehrer, Robert I.
Nature , Volume 301 (5902) – Feb 24, 1983
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References (16)

Publisher
Springer Journals
Copyright
Copyright © 1983 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/301715a0
Publisher site
See Article on Publisher Site

Abstract

On phagocytosing a microorganism, the neutrophil zpolymor-phonuclear leukocyte, PMN) consumes oxygen at a sharply elevated rate1. The oxygen is used to kill the microorganism, presumably being used to produce a potent oxidizing agent or agents. Candidates for these bactericidal agents are singlet oxygen, hydroxyl radical and chlorinating agents (that is, species containing ‘active’ Cl in a formal +1 oxidation state: HOCl, Cl2, N-chloroamides, and so on)1–5. We now report a semiquantitative assay for PMN-generated active chlorine based on its trapping with 1,3,5-trimethoxybenzene (TMB). Using this assay, we have found that at least 28% of the oxygen consumed by stimulated normal human PMNs is converted to active chlorinating agents.

Journal

Nature – Springer Journals

Published: Feb 24, 1983

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