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- Publisher
- Springer Journals
- Copyright
- Copyright © 2006 by Department of Plant Physiology
- Subject
- Life Sciences; Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture
- ISSN
- 0137-5881
- eISSN
- 1861-1664
- DOI
- 10.1007/s11738-006-0062-6
- Publisher site
- See Article on Publisher Site
Abstract
The cucumber cotyledon greening bioassay for cytokinins was modified by using 95% acetone-ethanol instead of 80% acetone as extraction solvent. The cotyledons were extracted directly with a 2:1 (v/v) acetone-ethanol solution in dark for 24 hours, omitting the homogenization and centrifugation operations of the previous bioassay. The modified bioassay is more convenient and especially useful in screening cytokinin-active substance from a large number of samples.
Journal
Acta Physiologiae Plantarum – Springer Journals
Published: Jun 14, 2006