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(2004)
BMC Microbiology BioMed Central Research article Identification and characterization of a nontypeable Haemophilus influenzae putative toxin-antitoxin locus
I. Karadžić, L. Izrael, G. Gojgić-Cvijović, Z. Vujčić (2002)
Leucine aminopeptidase from Streptomyces hygroscopicus is controlled by a low molecular weight inhibitor.Journal of bioscience and bioengineering, 94 4
R. Cahan, Efrat Hetzroni, M. Nisnevitch, Y. Nitzan (2007)
Purification and Identification of a Novel Leucine Aminopeptidase from Bacillus thuringiensis israelensisCurrent Microbiology, 55
T. Gonzalès, J. Robert‐Baudouy (1996)
Bacterial aminopeptidases: properties and functions.FEMS microbiology reviews, 18 4
E. Minagawa, S. Kaminogawa, H. Matsuzawa, T. Ohta, K. Yamauchi (1988)
Isolation and characterization of a thermostable aminopeptidase(aminopeptidase T) from Thermus aquaticus YT-1, an extremely thermophilic bacterium.Agricultural and biological chemistry, 52
R. Cahan, Itschak Axelrad, M. Safrin, D. Ohman, E. Kessler (2001)
A Secreted Aminopeptidase of Pseudomonas aeruginosaThe Journal of Biological Chemistry, 276
A. Shevchenko, M. Wilm, O. Vorm, Millard Mann (1996)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.Analytical chemistry, 68 5
S. Odintsov, I. Sabała, G. Bourenkov, V. Rybin, M. Bochtler (2005)
Substrate access to the active sites in aminopeptidase T, a representative of a new metallopeptidase clan.Journal of molecular biology, 354 2
R. Bargabus, N. Zidack, J. Sherwood, B. Jacobsen (2003)
Oxidative burst elicited by Bacillus mycoides isolate Bac J, a biological control agent, occurs independently of hypersensitive cell death in sugar beet.Molecular plant-microbe interactions : MPMI, 16 12
L. Kuo, G. Hwang, Yulan Lai, Shin-Ling Yang, Long-Liu Lin (2003)
Overexpression, Purification, and Characterization of the Recombinant Leucine Aminopeptidase II of Bacillus stearothermophilusCurrent Microbiology, 47
Y. Sanz, F. Toldrá (2002)
Purification and Characterization of an Arginine Aminopeptidase from Lactobacillus sakeiApplied and Environmental Microbiology, 68
L. Fanuel, C. Goffin, A. Cheggour, B. Devreese, G. Driessche, B. Joris, J. Beeumen, J. Frère (1999)
The DmpA aminopeptidase from Ochrobactrum anthropi LMG7991 is the prototype of a new terminal nucleophile hydrolase family.The Biochemical journal, 341 ( Pt 1)
S. Odintsov, I. Sabała, G. Bourenkov, V. Rybin, M. Bochtler (2005)
Staphylococcus aureus Aminopeptidase S Is a Founding Member of a New Peptidase Clan*Journal of Biological Chemistry, 280
Gang-Deog Lee, Sung-Sick Chun, Y. Kho, H. Chun (1998)
Purification and properties of an extracellular leucine aminopeptidase from the Bacillus sp. N2Journal of Applied Microbiology, 85
L. Kolehmainen, J. Mikola (1971)
Partial purification and enzymatic properties of an aminopeptidase from barley.Archives of biochemistry and biophysics, 145 2
H. Motoshima, N. Azuma, S. Kaminogawa, M. Ono, E. Minagawa, H. Matsuzawa, T. Ohta, K. Yamauchi (1990)
Molecular cloning and nucleotide sequence of the aminopeptidase T gene of Thermus aquaticus YT-1 and its high-level expression in Escherichia coli.Agricultural and biological chemistry, 54 9
M. Fernández-Esplá, F. Rul (1999)
PepS from Streptococcus thermophilus. A new member of the aminopeptidase T family of thermophilic bacteria.European journal of biochemistry, 263 2
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
T. Bolumar, Y. Sanz, M. Aristoy, F. Toldrá (2003)
Purification and properties of an arginyl aminopeptidase from Debaryomyces hansenii.International journal of food microbiology, 86 1-2
Long-Liu Lin, W. Hsu, Cheng-Pu Wu, M. Chi, Wei-Mou Chou, Hui‐Yu Hu (2004)
A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223Extremophiles, 8
B. Hansen, T. Leser, N. Hendriksen (2001)
Polymerase chain reaction assay for the detection of Bacillus cereus group cells.FEMS microbiology letters, 202 2
M. Matsui, J. Fowler, L. Walling (2006)
Leucine aminopeptidases: diversity in structure and function, 387
Long-Liu Lin, Yi-Pu Chen, Jia-Ci Yang, Y. Hua, Wen-Ching Wang, L. Kuo (2008)
Significance of the Conserved Tyr352 and Asp380 Residues in the Catalytic Activity of Bacillus stearothermophilus Aminopeptidase II as Evaluated by Site-directed MutagenesisThe Protein Journal, 27
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
J. Goldstein, Daniel Nelson, Tomasz Kordula, J. Mayo, J. Travis (2002)
Extracellular Arginine Aminopeptidase from Streptococcus gordonii FSS2Infection and Immunity, 70
Chung-Dar Lu (2006)
Pathways and regulation of bacterial arginine metabolism and perspectives for obtaining arginine overproducing strainsApplied Microbiology and Biotechnology, 70
U. Jankiewicz, W. Bielawski (2008)
Regulation of the activity of intracellular alanylaminopeptidase synthesized byPseudomonas sp.Folia Microbiologica, 47
An intracellular arginine—specific aminopeptidase synthesized by Bacillus mycoides was purified and characterized. The purification procedure for studied aminopeptidase consisted of ammonium sulphate precipitation and three chromatographic steps: anion exchange chromatography and gel permeation chromatography. A molecular weight of ∼50 kDa was estimated for the aminopeptidase by gel permeation chromatography and SDS-PAGE. The optimal activity of the enzyme on arginyl-β-naphthylamide as a substrate was at 37°C and pH 9.0. The enzyme showed maximum specificity for basic amino acids: such as Arg and Lys but was also able to hydrolyze aromatic amino acids: Trp, Tyr, and Phe. Co2+ ions activated the enzyme, while Zn2+, Cu2+, Hg2+ and Mn2+ inhibited it. The enzyme is a metalloaminopeptidase whose activity is inhibited by typical metalloaminopeptidase inhibitors: EDTA and 1,10-phenanthroline. Analysis of fragments of the amino acid sequence of the purified enzyme demonstrated high similarity to Amp S of Bacillus cereus and APII of B. thuringensis.
Applied Biochemistry and Microbiology – Springer Journals
Published: Mar 9, 2011
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