Alteration in the distribution of type IV collagen in glomerular basal laminae in diabetic rats as revealed by immunocytochemistry and morphometrical approach

Alteration in the distribution of type IV collagen in glomerular basal laminae in diabetic rats... 125 28 28 6 6 M. Bendayan Department of Anatomy, Faculty of Medicine University of Montreal Montreal Quebec Canada Summary The gomerular basal laminae of normoglycaemic and long-term streptozotocin-induced hyperglycaemic rats was studied by morphometrical and immunocytochemical approaches. Using the orthogonal intercept method, we have confirmed that in long-term diabetes, the thickness of the glomerular basal laminae increases significantly. Applying the high resolution protein A-gold immunocytochemical technique, type IV collagen was localized in the glomerular basal laminae. In tissues from normoglycaemic animals the labell ing was present over the central lamina densa. However, the labelling obtained over the thickened glomerular basal laminae of the hyperglycaemic animals was restricted to the subendothelial site of the lamina. Thus major alteration in the distributioa of type IV collagen occurs during the development of diabetic microangiopathy in hyperglycaemic animals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Diabetologia Springer Journals

Alteration in the distribution of type IV collagen in glomerular basal laminae in diabetic rats as revealed by immunocytochemistry and morphometrical approach

Diabetologia, Volume 28 (6) – Jun 1, 1985

Loading next page...
 
/lp/springer-journals/alteration-in-the-distribution-of-type-iv-collagen-in-glomerular-basal-06lDadI0nA
Publisher
Springer Journals
Copyright
Copyright © 1985 by Springer-Verlag
Subject
Medicine & Public Health; Human Physiology; Internal Medicine; Metabolic Diseases
ISSN
0012-186X
eISSN
1432-0428
DOI
10.1007/BF00283147
Publisher site
See Article on Publisher Site

Abstract

125 28 28 6 6 M. Bendayan Department of Anatomy, Faculty of Medicine University of Montreal Montreal Quebec Canada Summary The gomerular basal laminae of normoglycaemic and long-term streptozotocin-induced hyperglycaemic rats was studied by morphometrical and immunocytochemical approaches. Using the orthogonal intercept method, we have confirmed that in long-term diabetes, the thickness of the glomerular basal laminae increases significantly. Applying the high resolution protein A-gold immunocytochemical technique, type IV collagen was localized in the glomerular basal laminae. In tissues from normoglycaemic animals the labell ing was present over the central lamina densa. However, the labelling obtained over the thickened glomerular basal laminae of the hyperglycaemic animals was restricted to the subendothelial site of the lamina. Thus major alteration in the distributioa of type IV collagen occurs during the development of diabetic microangiopathy in hyperglycaemic animals.

Journal

DiabetologiaSpringer Journals

Published: Jun 1, 1985

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off