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Aerobic nitrate and nitrite reduction in continuous cultures of Escherichia coli E4

Aerobic nitrate and nitrite reduction in continuous cultures of Escherichia coli E4 203 153 153 6 6 Hans J. Brons Alexander J. B. Zehnder Department of Microbiology Wageningen Agricultural University Hesselink van Suchtelenweg 4 NL-6703 CT Wageningen The Netherlands Eurocetus B. V. Paasheuvelweg 30 NL-1105 BJ Amsterdam The Netherlands Abstract Nitrate and nitrite was reduced by Escherichia coli E4 in a l -lactate (5 mM) limited culture in a chemostat operated at dissolved oxygen concentrations corresponding to 90–100% air saturation. Nitrate reductase and nitrite reductase activity was regulated by the growth rate, and oxygen and nitrate concentrations. At a low growth rate (0.11 h −1 ) nitrate and nitrite reductase activities of 200 nmol · mg −1 protein · min −1 and 250 nmol · mg −1 protein · min −1 were measured, respectively. At a high growth rate (0.55 h −1 ) both enzyme activities were considerably lower (25 and 12 nmol mg −1 · protein · min −1 ). The steady state nitrite concentration in the chemostat was controlled by the combined action of the nitrate and nitrite reductase. Both nitrate and nitrite reductase activity were inversely proportional to the growth rate. The nitrite reductase activity decreased faster with growth rate than the nitrate reductase. The chemostat biomass concentration of E. coli E4, with ammonium either solely or combined with nitrate as a source of nitrogen, remained constant throughout all growth rates and was not affected by nitrite concentrations. Contrary to batch, E. coli E4 was able to grow in continuous cultures on nitrate as the sole source of nitrogen. When cultivated with nitrate as the sole source of nitrogen the chemostat biomass concentration is related to the activity of nitrate and nitrite reductase and hence, inversely proportional to growth rate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Microbiology Springer Journals

Aerobic nitrate and nitrite reduction in continuous cultures of Escherichia coli E4

Brons, Hans; Zehnder, Alexander
Archives of Microbiology , Volume 153 (6) – May 1, 1990
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References (35)

Publisher
Springer Journals
Copyright
Copyright © 1990 by Springer-Verlag
Subject
Life Sciences; Biotechnology; Biochemistry, general; Cell Biology; Ecology; Microbial Ecology; Microbiology
ISSN
0302-8933
eISSN
1432-072X
DOI
10.1007/BF00245261
Publisher site
See Article on Publisher Site

Abstract

203 153 153 6 6 Hans J. Brons Alexander J. B. Zehnder Department of Microbiology Wageningen Agricultural University Hesselink van Suchtelenweg 4 NL-6703 CT Wageningen The Netherlands Eurocetus B. V. Paasheuvelweg 30 NL-1105 BJ Amsterdam The Netherlands Abstract Nitrate and nitrite was reduced by Escherichia coli E4 in a l -lactate (5 mM) limited culture in a chemostat operated at dissolved oxygen concentrations corresponding to 90–100% air saturation. Nitrate reductase and nitrite reductase activity was regulated by the growth rate, and oxygen and nitrate concentrations. At a low growth rate (0.11 h −1 ) nitrate and nitrite reductase activities of 200 nmol · mg −1 protein · min −1 and 250 nmol · mg −1 protein · min −1 were measured, respectively. At a high growth rate (0.55 h −1 ) both enzyme activities were considerably lower (25 and 12 nmol mg −1 · protein · min −1 ). The steady state nitrite concentration in the chemostat was controlled by the combined action of the nitrate and nitrite reductase. Both nitrate and nitrite reductase activity were inversely proportional to the growth rate. The nitrite reductase activity decreased faster with growth rate than the nitrate reductase. The chemostat biomass concentration of E. coli E4, with ammonium either solely or combined with nitrate as a source of nitrogen, remained constant throughout all growth rates and was not affected by nitrite concentrations. Contrary to batch, E. coli E4 was able to grow in continuous cultures on nitrate as the sole source of nitrogen. When cultivated with nitrate as the sole source of nitrogen the chemostat biomass concentration is related to the activity of nitrate and nitrite reductase and hence, inversely proportional to growth rate.

Journal

Archives of Microbiology – Springer Journals

Published: May 1, 1990

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