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Absence or presence of glucose in growth medium and its effect on heat injury in Staphylococcus aureus

Absence or presence of glucose in growth medium and its effect on heat injury in Staphylococcus... Staphylococcus aureus 196E, when grown in a glucose (≥0.25% wt./vol.)-containing medium, produced cells that would undergo injury when subjected to sublethal heat conditions (45 min at 50°C); however, if glucose was omitted from the growth medium, the extent of injury was greatly reduced. Media containing glucose sterilized by filtration or by separate autoclaving produced cells equal in injury susceptibility to medium in which glucose was autoclaved as part of the medium components. Injury also occurred when other sugars such as fructose, mannose, maltose, or lactose were substituted for glucose. Sugar-containing media that produced Staphylococcus aureus of maximal susceptibility to heat injury reached a pH of approximately 6 or lower during growth of the cells. Incubation of staphylococci in growth medium acidified with acetic or lactic acids or HCl did not lead to cells that would undergo injury under the stated conditions. The stimulatory effect of glucose on injury appears to be related to the metabolism of the sugar by Staphylococcus aureus . http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Industrial Microbiology Biotechnology Springer Journals

Absence or presence of glucose in growth medium and its effect on heat injury in Staphylococcus aureus

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References (15)

Publisher
Springer Journals
Copyright
Copyright © 1986 by Society for Industrial Microbiology
Subject
Chemistry; Biotechnology; Genetic Engineering; Biochemistry, general; Bioinformatics; Microbiology; Microbial Genetics and Genomics
ISSN
1367-5435
eISSN
1476-5535
DOI
10.1007/BF01569314
Publisher site
See Article on Publisher Site

Abstract

Staphylococcus aureus 196E, when grown in a glucose (≥0.25% wt./vol.)-containing medium, produced cells that would undergo injury when subjected to sublethal heat conditions (45 min at 50°C); however, if glucose was omitted from the growth medium, the extent of injury was greatly reduced. Media containing glucose sterilized by filtration or by separate autoclaving produced cells equal in injury susceptibility to medium in which glucose was autoclaved as part of the medium components. Injury also occurred when other sugars such as fructose, mannose, maltose, or lactose were substituted for glucose. Sugar-containing media that produced Staphylococcus aureus of maximal susceptibility to heat injury reached a pH of approximately 6 or lower during growth of the cells. Incubation of staphylococci in growth medium acidified with acetic or lactic acids or HCl did not lead to cells that would undergo injury under the stated conditions. The stimulatory effect of glucose on injury appears to be related to the metabolism of the sugar by Staphylococcus aureus .

Journal

Journal of Industrial Microbiology BiotechnologySpringer Journals

Published: Jun 1, 1986

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