A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a soybean cultivar carrying Rsv1 and Rsv3

A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a... It is generally believed that infidelity of RNA virus replication combined with R -gene-driven selection is one of the major evolutionary forces in overcoming host resistance. In this study, we utilized an avirulent soybean mosaic virus (SMV) mutant to examine the possibility of emergence of mutant viruses capable of overcoming R -gene-mediated resistance during serial passages. Interestingly, we found that the emerged progeny virus induced severe rugosity and local necrotic lesions in Jinpumkong-2 ( Rsv1 + Rsv3 ) plants, while SMV-G7H provoked a lethal systemic hypersensitive response. Genome sequence analysis of the emerged progeny virus revealed that the mutation in CI that had caused SMV-G7H to lose its virulence was restored to the original sequence, and a single amino acid was newly introduced into HC-Pro, which means that the symptom alteration was due to this single amino acid mutation in HC-Pro. Our results suggest that SMV HC-Pro functions as a symptom determinant in the SMV-soybean pathosystem. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A single amino acid change in HC-Pro of soybean mosaic virus alters symptom expression in a soybean cultivar carrying Rsv1 and Rsv3

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0829-3
Publisher site
See Article on Publisher Site

Abstract

It is generally believed that infidelity of RNA virus replication combined with R -gene-driven selection is one of the major evolutionary forces in overcoming host resistance. In this study, we utilized an avirulent soybean mosaic virus (SMV) mutant to examine the possibility of emergence of mutant viruses capable of overcoming R -gene-mediated resistance during serial passages. Interestingly, we found that the emerged progeny virus induced severe rugosity and local necrotic lesions in Jinpumkong-2 ( Rsv1 + Rsv3 ) plants, while SMV-G7H provoked a lethal systemic hypersensitive response. Genome sequence analysis of the emerged progeny virus revealed that the mutation in CI that had caused SMV-G7H to lose its virulence was restored to the original sequence, and a single amino acid was newly introduced into HC-Pro, which means that the symptom alteration was due to this single amino acid mutation in HC-Pro. Our results suggest that SMV HC-Pro functions as a symptom determinant in the SMV-soybean pathosystem.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2011

References

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