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Biotechnol Lett (2018) 40:1109–1118 https://doi.org/10.1007/s10529-018-2560-1 ORIGINAL RESEARCH PAPER A novel malic enzyme gene, Mime2, from Mortierella isabellina M6-22 contributes to lipid accumulation . . . . . Shan Li Lingyan Li Xiangfeng Xiong Xiuling Ji Yunlin Wei Lianbing Lin Qi Zhang Received: 27 February 2018 / Accepted: 27 April 2018 / Published online: 18 May 2018 Springer Science+Business Media B.V., part of Springer Nature 2018 Abstract enzymatic activity of MIME2 is 177.46 U/mg, and the Objective This study was aimed at cloning and K and V for NADP are 0.703 mM and m max characterizing a novel malic enzyme (ME) gene of 156.25 lg/min, respectively. Besides, Mime2 trans- Mortierella isabellina M6-22 and identifying its formation significantly increased the cell lipid content relation with lipid accumulation. in strain YM25235 (3.15 ± 0.24 vs. 2.17 ± 0.31 g/L, Methods Mime2 was cloned from strain M6-22. p \ 0.01). Plasmid pET32aMIME2 was constructed to express Conclusions The novel ME gene Mime2 isolated ME of MIME2 in Escherichia coli BL21. After from strain M6-22 contributes to lipid accumulation in purification, the optimal pH and temperature of strain YM25235. MIME2, as well as K and V for NADP were m max determined. The effects
Biotechnology Letters – Springer Journals
Published: May 18, 2018
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