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R. Jenkins, E. Wilson, R. Angus, W. Howell, M. Kirk (2003)
Androstenedione and progesterone in the sediment of a river receiving paper mill effluent.Toxicological sciences : an official journal of the Society of Toxicology, 73 1
(2004)
Proc Natl Acad Sci
M. Nielen, T. Bovee, M. Engelen, P. Rutgers, A. Hamers, J. Rhijn, L. Hoogenboom (2006)
Urine testing for designer steroids by liquid chromatography with androgen bioassay detection and electrospray quadrupole time-of-flight mass spectrometry identification.Analytical chemistry, 78 2
(2005)
Biosens Bioelectron
(2004)
Toxicol Appl Pharmacol
(2006)
Anal Bioanal Chem
L. Hoogenboom, L. Haan, D. Hooijerink, G. Bor, A. Murk, A. Brouwer (2001)
Estrogenic activity of estradiol and its metabolites in the ER‐CALUX assay with human T47D breast cells NoteAPMIS, 109
(1999)
Sci Total Environ
Derek Henley, Natasha Lipson, K. Korach, C. Bloch (2007)
Prepubertal gynecomastia linked to lavender and tea tree oils.The New England journal of medicine, 356 5
(2003)
Biochemistry
(1999)
Chemosphere
D. Collins (1994)
Sex hormone receptor binding, progestin selectivity, and the new oral contraceptives.American journal of obstetrics and gynecology, 170 5 Pt 2
(1993)
Epidemiol Rev
(2006)
Van der Burg B Toxicol Sci
(2005)
J Mol Endocrinol
S. Mueller (2002)
Overview of in vitro tools to assess the estrogenic and antiestrogenic activity of phytoestrogens.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 777 1-2
Hilda Witters, C. Vangenechten, P. Berckmans (2001)
Detection of estrogenic activity in Flemish surface waters using an in vitro recombinant assay with yeast cells.Water science and technology : a journal of the International Association on Water Pollution Research, 43 2
(1996)
Mol Cell Biol
(2006)
Selected TitlesAlternatives to Laboratory Animals, 34
U. Fuhrmann, R. Krattenmacher, E. Slater, K. Fritzemeier (1996)
The novel progestin drospirenone and its natural counterpart progesterone: biochemical profile and antiandrogenic potential.Contraception, 54 4
E. Sonneveld, J. Riteco, Hendrina Jansen, B. Pieterse, A. Brouwer, W. Schoonen, B. Burg (2006)
Comparison of in vitro and in vivo screening models for androgenic and estrogenic activities.Toxicological sciences : an official journal of the Society of Toxicology, 89 1
A. Haelens, G. Verrijdt, L. Callewaert, V. Christiaens, K. Schauwaers, B. Peeters, W. Rombauts, F. Claessens (2003)
DNA recognition by the androgen receptor: evidence for an alternative DNA-dependent dimerization, and an active role of sequences flanking the response element on transactivation.The Biochemical journal, 369 Pt 1
K. Rehmann, Karl-Werner Schramm, Antonius Kettrup (1999)
Applicability of a yeast oestrogen screen for the detection of oestrogen-like activities in environmental samples.Chemosphere, 38 14
K. Gaido, L. Leonard, Stephanie Lovell, J. Gould, Dariouche Babaï, C. Portier, D. McDonnell (1997)
Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay.Toxicology and applied pharmacology, 143 1
(2007)
N Engl J Med
C. Peteghem (2007)
Papers presented at the 5th International Symposium on Hormone and Veterinary Drug Residue Analysis - Antwerp, Belgium 16-19 May 2006 - PrefaceAnalytica Chimica Acta, 586
(2001)
Acta Pathol Microbiol Immunol Scand
T. Bovee, G. Bor, H. Heskamp, R. Hoogenboom, M. Nielen (2006)
Validation and application of a robust yeast estrogen bioassay for the screening of estrogenic activity in animal feedFood Additives & Contaminants, 23
T. Bovee, Richard Helsdingen, P. Koks, H. Kuiper, R. Hoogenboom, J. Keijer (2004)
Development of a rapid yeast estrogen bioassay, based on the expression of green fluorescent protein.Gene, 325
P. Willemsen, M. Scippo, G. Maghuin-Rogister, J. Martial, M. Muller (2005)
Enhancement of steroid receptor-mediated transcription for the development of highly responsive bioassaysAnalytical and Bioanalytical Chemistry, 382
T. Bovee, Richard Helsdingen, I. Rietjens, J. Keijer, R. Hoogenboom (2004)
Rapid yeast estrogen bioassays stably expressing human estrogen receptors α and β, and green fluorescent protein: a comparison of different compounds with both receptor typesThe Journal of Steroid Biochemistry and Molecular Biology, 91
E. Michelini, Piia Leskinen, M. Virta, M. Karp, A. Roda (2005)
A new recombinant cell-based bioluminescent assay for sensitive androgen-like compound detection.Biosensors & bioelectronics, 20 11
P. Braverman, V. Strasburger (1924)
ContraceptionThe Indian Medical Gazette, 59
N. Garcia-Reyero, Eulàlia Grau, M. Castillo, M. Alda, D. Barceló, B. Piña (2001)
Monitoring of endocrine disruptors in surface waters by the yeast recombinant assayEnvironmental Toxicology and Chemistry, 20
W. Schoonen, G. Deckers, M.E Gooijer, R. Ries, H. Kloosterboer (2000)
Hormonal properties of norethisterone, 7α-methyl-norethisterone and their derivativesThe Journal of Steroid Biochemistry and Molecular Biology, 74
G. Lemaire, B. Térouanne, P. Mauvais, S. Michel, R. Rahmani (2004)
Effect of organochlorine pesticides on human androgen receptor activation in vitro.Toxicology and applied pharmacology, 196 2
K. Graumann, A. Breithofer, Alois Jungbauer (1999)
Monitoring of estrogen mimics by a recombinant yeast assay: synergy between natural and synthetic compounds?The Science of the total environment, 225 1-2
P. Shaffer, A. Jivan, Eric Dollins, F. Claessens, D. Gewirth (2004)
Structural basis of androgen receptor binding to selective androgen response elements.Proceedings of the National Academy of Sciences of the United States of America, 101 14
(1995)
Environ Health Perspect
N. Skakkebaek, N. Jørgensen, K. Main, E. Meyts, H. Leffers, A. Andersson, A. Juul, E. Carlsen, G. Mortensen, T. Jensen, J. Toppari (2006)
Is human fecundity declining?International journal of andrology, 29 1
S. Jobling, T. Reynolds, Roger White, Malcolm Parker, J. Sumpter (1995)
A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic.Environmental Health Perspectives, 103
(2003)
In Vitro Toxicol
M. Pike, D. Spicer, L. Dahmoush, M. Press (1993)
Estrogens, progestogens, normal breast cell proliferation, and breast cancer risk.Epidemiologic reviews, 15 1
S. Eykyn (1950)
MicrobiologyThe Yale Journal of Biology and Medicine, 22
B. Blankvoort, E. Groene, A. Meeteren-Kreikamp, R. Witkamp, R. Rodenburg, J. Aarts (2001)
Development of an androgen reporter gene assay (AR-LUX) utilizing a human cell line with an endogenously regulated androgen receptor.Analytical biochemistry, 298 1
Jacqueline Brodie, I. McEwan (2005)
Intra-domain communication between the N-terminal and DNA-binding domains of the androgen receptor: modulation of androgen response element DNA binding.Journal of molecular endocrinology, 34 3
(2006)
Int J Androl
(2002)
J Chromatogr B
B. Cormack, G. Bertram, M. Egerton, N. Gow, S. Falkow, S. Falkow, A. Brown (1997)
Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans.Microbiology, 143 ( Pt 2)
Hyun Lee, Y. Lee, H. Kwon, Keesook Lee (2003)
Novel yeast bioassay system for detection of androgenic and antiandrogenic compounds.Toxicology in vitro : an international journal published in association with BIBRA, 17 2
(2001)
Water Sci Technol
Markus Imhof, D. McDonnell (1996)
Yeast RSP5 and its human homolog hRPF1 potentiate hormone-dependent activation of transcription by human progesterone and glucocorticoid receptorsMolecular and Cellular Biology, 16
(2006)
Food Addit Contam
W. Dhooge, K. Arijs, I. D'haese, S. Stuyvaert, B. Versonnen, Colin Janssen, W. Verstraete, F. Comhaire (2006)
Experimental parameters affecting sensitivity and specificity of a yeast assay for estrogenic compounds: results of an interlaboratory validation exerciseAnalytical and Bioanalytical Chemistry, 386
(2004)
J Steroid Biochem Mol Biol
(1994)
Am J Obstet Gynecol
Public concern about the presence of natural and anthropogenic compounds which affect human health by modulating normal endocrine functions is continuously growing. Fast and simple high-throughput screening methods for the detection of hormone activities are thus indispensable. During the last two decades, a panel of different in vitro assays has been developed, mainly for compounds with an estrogenic mode of action. Here we describe the development of an androgen transcription activation assay that is easy to use in routine screening. Recombinant yeast cells were constructed that express the human androgen receptor and yeast enhanced green fluorescent protein (yEGFP), the latter in response to androgens. Compared with other reporters, the yEGFP reporter protein is very convenient because it is directly measurable in intact living cells, i.e., cell wall disruption and the addition of a substrate are not needed. When yeast was exposed to 17β-testosterone, the concentration where half-maximal activation is reached (EC50) was 50 nM. The relative androgenic potencies, defined as the ratio between the EC50 of 17β-testosterone and the EC50 of the compound, of 5α-dihydrotestosterone, methyltrienolone, and 17β-boldenone are 2.3, 1.4, and 0.15 respectively. The results presented in this paper demonstrate that this new yeast androgen bioassay is fast, sensitive, and very specific and also suited to detect compounds that have an antiandrogenic mode of action.
Analytical and Bioanalytical Chemistry – Springer Journals
Published: Sep 12, 2007
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