A human gut microbial gene catalogue established by metagenomic sequencing

A human gut microbial gene catalogue established by metagenomic sequencing To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, ∼150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

A human gut microbial gene catalogue established by metagenomic sequencing

Qin, Junjie; Li, Ruiqiang; Raes, Jeroen; Arumugam, Manimozhiyan; Burgdorf, Kristoffer Solvsten; Manichanh, Chaysavanh; Nielsen, Trine; Pons, Nicolas; Levenez, Florence; Yamada, Takuji; Mende, Daniel R.; Li, Junhua; Xu, Junming; Li, Shaochuan; Li, Dongfang; Cao, Jianjun; Wang, Bo; Liang, Huiqing; Zheng, Huisong; Xie, Yinlong; Tap, Julien; Lepage, Patricia; Bertalan, Marcelo; Batto, Jean-Michel; Hansen, Torben; Le Paslier, Denis; Linneberg, Allan; Nielsen, H. Bjørn; Pelletier, Eric; Renault, Pierre; Sicheritz-Ponten, Thomas; Turner, Keith; Zhu, Hongmei; Yu, Chang; Li, Shengting; Jian, Min; Zhou, Yan; Li, Yingrui; Zhang, Xiuqing; Li, Songgang; Qin, Nan; Yang, Huanming; Wang, Jian; Brunak, Søren; Doré Joel; Guarner, Francisco; Kristiansen, Karsten; Pedersen, Oluf; Parkhill, Julian; Weissenbach, Jean; Antolin, Maria; Artiguenave, François; Blottiere, Hervé; Borruel, Natalia; Bruls, Thomas; Casellas, Francesc; Chervaux, Christian; Cultrone, Antonella; Delorme, Christine; Denariaz, Gérard; Dervyn, Rozenn; Forte, Miguel; Friss, Carsten; van de Guchte, Maarten; Guedon, Eric; Haimet, Florence; Jamet, Alexandre; Juste, Catherine; Kaci, Ghalia; Kleerebezem, Michiel; Knol, Jan; Kristensen, Michel; Layec, Severine; Le Roux, Karine; Leclerc, Marion; Maguin, Emmanuelle; Melo Minardi, Raquel; Oozeer, Raish; Rescigno, Maria; Sanchez, Nicolas; Tims, Sebastian; Torrejon, Toni; Varela, Encarna; de Vos, Willem; Winogradsky, Yohanan; Zoetendal, Erwin; Bork, Peer; Ehrlich, S. Dusko; Wang, Jun
Nature, Volume 464 (7285) – Mar 4, 2010

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Publisher
Springer Journals
Copyright
Copyright © 2010 Macmillan Publishers Limited. All rights reserved
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/nature08821
pmid
20203603
Publisher site
See Article on Publisher Site

Abstract

To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, ∼150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

Journal

NatureSpringer Journals

Published: Mar 4, 2010

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