A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples

A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue... Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method’s potential applications in routine veterinary quarantine and epidemic surveillance of FMDV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

A highly sensitive and specific multiplex RT-PCR to detect foot-and-mouth disease virus in tissue and food samples

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Publisher
Springer Journals
Copyright
Copyright © 2008 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-007-1082-y
Publisher site
See Article on Publisher Site

Abstract

Three sets of primers to detect foot-and-mouth disease virus (FMDV) using multiplex RT-PCR were designed based on several reference nucleotide sequences, and their reaction conditions were determined. By testing ten-fold serial dilutions of FMDV, the sensitivity of multiplex RT-PCR is 100 times higher than conventional RT-PCR. Meanwhile, its specificity was confirmed compared with other related vesicular disease viruses. Furthermore, 30 field samples from different animals were tested, and the results supported the method’s potential applications in routine veterinary quarantine and epidemic surveillance of FMDV.

Journal

Archives of VirologySpringer Journals

Published: Jan 1, 2008

References

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