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A Computer Scientist's Guide to Cell BiologyOther Ways to Use Biology for Biological Experiments

A Computer Scientist's Guide to Cell Biology: Other Ways to Use Biology for Biological Experiments [There are a number of reasons for wanting to insert foreign DNA into a cell, one of which is simply to amplify (increase) the quantity of foreign DNA by making use of a cell’s natural ability to grow and multiply. However, there is a more direct way to amplify DNA, by using of some of the cell’s DNA replication machinery in vitro. This technique is called polymerase chain reaction (PCR). To explain how it works, I will first review, at a high level, how DNA is duplicated (replicated) in a cell. (The mechanisms for this differ somewhat in prokaryotes and eukaryotes—here I will focus on prokaryotic replication).] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Computer Scientist's Guide to Cell BiologyOther Ways to Use Biology for Biological Experiments

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Publisher
Springer US
Copyright
© Springer Science+Business Media, LLC 2007
ISBN
978-0-387-48275-0
Pages
71 –81
DOI
10.1007/978-0-387-48278-1_6
Publisher site
See Chapter on Publisher Site

Abstract

[There are a number of reasons for wanting to insert foreign DNA into a cell, one of which is simply to amplify (increase) the quantity of foreign DNA by making use of a cell’s natural ability to grow and multiply. However, there is a more direct way to amplify DNA, by using of some of the cell’s DNA replication machinery in vitro. This technique is called polymerase chain reaction (PCR). To explain how it works, I will first review, at a high level, how DNA is duplicated (replicated) in a cell. (The mechanisms for this differ somewhat in prokaryotes and eukaryotes—here I will focus on prokaryotic replication).]

Published: Jan 1, 2007

Keywords: Foreign Substance; Lagging Strand; Polymerase Chain Reaction Process; Copying Procedure; Complete Polymerase Chain Reaction

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