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C. Kreader (1998)
Persistence of PCR-Detectable Bacteroides distasonis from Human Feces in River WaterApplied and Environmental Microbiology, 64
L. Sinton, R. Finlay, D. Hannah (1998)
Distinguishing human from animal faecal contamination in water: A reviewNew Zealand Journal of Marine and Freshwater Research, 32
H. Smith, M. Linggood (1971)
Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea.Journal of medical microbiology, 4 4
B. Green, R. Neill, W. Ruyechan, R. Holmes (1983)
Evidence that a new enterotoxin of Escherichia coli which activates adenylate cyclase in eucaryotic target cells is not plasmid mediatedInfection and Immunity, 41
K. Krogfelt (1991)
Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli.Reviews of infectious diseases, 13 4
C. Pickett, D. Weinstein, R. Holmes (1987)
Genetics of type IIa heat-labile enterotoxin of Escherichia coli: operon fusions, nucleotide sequence, and hybridization studiesJournal of Bacteriology, 169
S. Parveen, K. Portier, Kevin Robinson, L. Edmiston, M. Tamplin (1999)
Discriminant Analysis of Ribotype Profiles of Escherichia coli for Differentiating Human and Nonhuman Sources of Fecal PollutionApplied and Environmental Microbiology, 65
A. Dufour, E. Strickland, V. Cabelli (1981)
Membrane filter method for enumerating Escherichia coliApplied and Environmental Microbiology, 41
P. Nichols, R. Leeming, M. Rayner, V. Latham (1996)
Use of capillary gas chromatography for measuring fecal-derived sterols application to stormwater, the sea-surface microlayer, beach greases, regional studies, and distinguishing algal blooms and human and non-human sources of sewage pollutionJournal of Chromatography A, 733
T. Besser (1994)
Escherichia coli septicaemia in calves
W. Grabow, T. Neubrech, C. Holtzhausen, J. Jofre (1995)
Bacteroides fragilis and Escherichia coli bacteriophages: excretion by humans and animalsWater Science and Technology, 31
S. Stacy-Phipps, James Mecca, J. Weiss (1995)
Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infectionJournal of Clinical Microbiology, 33
(1994)
Diarrhoea and dysentery in calves
R. Feachem (1975)
An improved role for faecal coliform to faecal streptococci ratios in the differentiation between human and non-human pollution sourcesWater Research, 9
S. Altschul, Thomas Madden, A. Schäffer, Jinghui Zhang, Zheng Zhang, W. Miller, D. Lipman (1997)
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic acids research, 25 17
S. Parveen, R. Murphree, L. Edmiston, C. Kaspar, K. Portier, M. Tamplin (1997)
Association of multiple-antibiotic-resistance profiles with point and nonpoint sources of Escherichia coli in Apalachicola BayApplied and Environmental Microbiology, 63
C. Hagedorn, S. Robinson, Jennifer Filtz, Sarah Grubbs, Theresa Angier, R. Reneau (1999)
Determining Sources of Fecal Pollution in a Rural Virginia Watershed with Antibiotic Resistance Patterns in Fecal StreptococciApplied and Environmental Microbiology, 65
M. Pommepuy, M. Butin, A. Derrien, M. Gourmelon, R. Colwell, M. Cormier (1996)
Retention of enteropathogenicity by viable but nonculturable Escherichia coli exposed to seawater and sunlightApplied and Environmental Microbiology, 62
M. Bahirathan, L. Puente, P. Seyfried (1998)
Use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution.Canadian journal of microbiology, 44 11
Y. Tsai, B. Olson (1992)
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reactionApplied and Environmental Microbiology, 58
(1998)
DNA Library World Give Investigators Inside Poop on Pollution Sources
(1997)
Occurrence of STh toxin gene in wastewater
P. Servais, G. Billen, J. Rego (1985)
Rate of Bacterial Mortality in Aquatic EnvironmentsApplied and Environmental Microbiology, 49
H. Arie, Havelaar, M. van, Olphen, Y. Drost (1993)
F-specific RNA bacteriophages are adequate model organisms for enteric viruses in fresh waterApplied and Environmental Microbiology, 59
A. Bernhard, K. Field (2000)
Identification of Nonpoint Sources of Fecal Pollution in Coastal Waters by Using Host-Specific 16S Ribosomal DNA Genetic Markers from Fecal AnaerobesApplied and Environmental Microbiology, 66
This research describes a method based on PCR to identify cattle fecal pollution in water using a portion of the heat labile toxin IIA (LTIIa) gene from enterotoxigenic Escherichia coli (ETEC). We describe the development of the primers and target. DNA extracts (221) from different animal fecal and human sewage samples were screened and showed no cross-reactivity. Minimum detection limits using centrifugation and filtration methods to concentrate E. coli seeded into stream, ocean, and secondary effluent waters were found to be at femtogram and attogram levels, respectively. Stability of the biomarker in stream, ocean, and secondary effluent waters was 2–4 weeks for all water types. Finally, 33 farm lagoon and waste samples were collected and 31 tested to validate the method; 93% were positive for the LTIIa trait when >1,000 E. coli were screened and 100% positive when >105 E. coli were screened. Prevalence of the toxin gene in the E. coli population affected the outcome of the analyses. The cow biomarker can be used in watershed studies to identify cattle waste with great accuracy if the appropriate numbers of E. coli are screened.
Applied Microbiology and Biotechnology – Springer Journals
Published: Jun 16, 2002
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