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- Publisher
- Springer Journals
- Copyright
- Copyright © 2009 by Springer Science+Business Media, LLC
- Subject
- Engineering; Engineering Fluid Dynamics; Nanotechnology; Biophysics and Biological Physics; Biomedical Engineering
- ISSN
- 1387-2176
- eISSN
- 1572-8781
- DOI
- 10.1007/s10544-009-9333-5
- pmid
- 19578992
- Publisher site
- See Article on Publisher Site
Abstract
This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.
Journal
Biomedical Microdevices – Springer Journals
Published: Jul 4, 2009