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Examination of fungal stress response genes using Saccharomyces cerevisiae as a model system: targeting genes affecting aflatoxin biosynthesis by Aspergillus flavus Link



Saccharomyces cerevisiae served as a model fungal system to examine functional genomics of oxidative stress responses and reactions to test antioxidant compounds. Twenty-two strains of S. cerevisiae , including a broad spectrum of singular gene deletion mutants, were exposed to hydrogen peroxide (H 2 O 2 ) to examine phenotypic response to oxidative stress. Responses of particular mutants treated with gallic, tannic or caffeic acids, or methyl gallate, during H 2 O 2 exposure, indicated that these compounds alleviated oxidative stress. These compounds are also potent inhibitors of aflatoxin biosynthesis in Aspergillus flavus . To gain further insights into a potential link between oxidative stress and aflatoxin biosynthesis, 43 orthologs of S. cerevisiae genes involved in gene regulation, signal transduction (e.<?A3B2 show $132#?>g., SHO1 , HOG1 , etc.) and antioxidation (e.<?A3B2 show $132#?>g., CTT1 , CTA1 , etc.) were identified in an A. flavus expressed sequence tag library. A successful exemplary functional complementation of an antioxidative stress gene from A. flavus , mitochondrial superoxide dismutase ( sodA ), in a sod2 Δ yeast mutant further supported the potential of S. cerevisiae deletion mutants to serve as a model system to study A. flavus . Use of this system to further examine functional genomics of oxidative stress in aflatoxigenesis and reduction of aflatoxin biosynthesis by antioxidants is discussed.



Applied Microbiology and BiotechnologySpringer Journals

Published: Jun 1, 2005

DOI: 10.1007/s00253-004-1821-1

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