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Evaluation of a Human Bio-Engineered Skin Equivalent for Drug Permeation Studies



Purpose . To test the barrier function of a bio-engineered human skin (BHS) using three model drugs (caffeine, hydrocortisone, and tamoxifen) in vitro . To investigate the lipid composition and microscopic structure of the BHS. Methods . The human skin substitute was composed of both epidermal and dermal layers, the latter having a bovine collagen matrix. The permeability of the BHS to three model drugs was compared to that obtained in other percutaneous testing models (human cadaver skin, hairless mouse skin, and EpiDerm™). Lipid analysis of the BHS was performed by high performance thin layered chromatrography. Histological evalulation of the BHS was performed using routine H&E staining. Results . The BHS mimicked human skin in terms of lipid composition, gross ultrastructure, and the formation of a stratum corneum. However, the permeability of the BHS to caffeine, hydrocortisone, and tamoxifen was 3-4 fold higher than that of human cadaver skin. Conclusions . In summary, the results indicate that the BHS may be an acceptable in vitro model for drug permeability testing.



Pharmaceutical ResearchSpringer Journals

Published: Sep 1, 2000

DOI: 10.1023/A:1026405712870

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