In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography

In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection... We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo , 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biomedical Optics SPIE

In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography

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Publisher
SPIE
Copyright
Copyright © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)
ISSN
1083-3668
eISSN
1560-2281
D.O.I.
10.1117/1.3640808
Publisher site
See Article on Publisher Site

Abstract

We introduce flow optical projection tomography, an imaging technique capable of visualizing the vasculature of living specimens in 3-D. The method detects the movement of cells in the bloodstream and creates flow maps using a motion-analysis procedure. Then, flow maps obtained from projection taken at several angles are used to reconstruct sections of the circulatory system of the specimen. We therefore demonstrate an in vivo , 3-D optical imaging technique that, without the use of any labeling, is able to reconstruct and visualize the vascular network of transparent and weakly scattering living specimens.

Journal

Journal of Biomedical OpticsSPIE

Published: Oct 1, 2011

Keywords: biomechanics; biomedical optical imaging; cellular biophysics; haemodynamics; image reconstruction; medical image processing; optical tomography; optical tomography; vascular networks; label free

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