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, and the silencing effects were confirmed by western blotting assays (Figure 3A). CCK8 and BrdU assays showed that knockdown of ZC3H14 significantly promoted the cells growth and proliferation of both HepG2 and MHCC ...
into clear-bottom, 96-well plates (black-sided for fluorescence assays ) at a concentration of 4 × 104 cells per well in DMEM without phenol red and allowed to grow for 24 h before dosing. For multiplex ...
Expression Changes of the PLD Biomarkers and Corresponding PI: Second Experiment ( HepG2 cells exposed to a range of compound concentrations) View Large Cytotoxicity Determination No cytotoxicity assays ...
to identify signal transduction pathways predominantly involved in the regulation of gene transcription by DILI drugs. To this end, we employed ATTAGENE’s cell -based multiplexed reporter assay , the FACTORIAL ...
was detected at 594 nm, while Hoechst was excited at 405 nm and its emission was detected at 490 nm. Microplate assay . HepG2 cells were seeded into 96-well plates at a density of 3 × 104 cells per well ...
standard deviations of individual microwell AI values from duplicate assays using 2 different devices (n > 1800 per condition). PMA-induced protease activity of single HepG2 cells As one of the most common ...
(CellSensor ARE-bla HepG2 cell line; K1633; Thermo Fisher Scientific) [41]. This assay was multiplexed with the CellTiter Glo assay (G7570; Promega, Madison, WI, http://www.promega.com) to assess compound ...
or downregulated in liver cell lines, which may be involved in endothelial cell leakage in the context of CCHFV. Methods Virus and cells HepG2 and Huh7 are well-differentiated hepatocarcinoma cell lines derived from ...
. The growth rates were analysed by MTS assay . The data represent the results of three independent experiments. (C) The colony formation abilities of differently transfected HepG2 and Huh7 cells . The cell foci ...
), and reverse transcription (Fig 1B), like the human hepatoma HepG2 cell line. To determine if these two woodchuck cell lines could support HBV cccDNA formation, we analyzed the Hirt DNA extracts from ...
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