Ultrasmall endogenous biopolymer nanoparticles for magnetic resonance/photoacoustic dual-modal imaging-guided photothermal therapyElectronic supplementary information (ESI) available: FT-IR spectra of MNP, PEG-MNP and NH2-PEG5000-NH2 (Fig. S1). Relationship of MNP-PEG with the amine group determined by fluorescamine (Fig. S2). Mn2+ stability study of MNP-Mn in PBS (pH = 7.4) and acidic buffer solution (pH = 5.5) (Fig. S3). Fig. S4 (a) photos of MNP-Mn in PBS and serum with different incubation time. (b) Hydrodynamic size distribution of the MNP-Mn during different incubation periods with serum, suggesting good stability in serum. Temperature curves of MNP-Mn with the same concentration (200 g mL1) and PBS irradiated by an 808 nm laser at different laser power density for 5 min (Fig. S5). Fig. S6 (a) temperature curves of MNP-Mn, ICG solutions and water under five cycles of photothermal heating by an 808 nm laser. (b) Photos of MNP-Mn, ICG solutions and water before and after photothermal heating. Linear time data versus ln() obtained from the cooling period (Fig. S7). Cell viabilities of the NIH 3T3 cells after 24 h incubation with MNP-Mn at various concentrations by MTT assay (Fig. S8). UV-Vis and fluorescence spectra of rhodamine and MNP-Mn-rhodamine solutions (Fig. S9). T1-Weighted MR coronal images after i.t. injection into the tumor bearing mice at 24 h and 5 day using 3.0 T clinical MRI equipment (Fig. S10). See DOI: 10.1039/c8nr01215f

Ultrasmall endogenous biopolymer nanoparticles for magnetic resonance/photoacoustic dual-modal... Multi-modal imaging-guided photothermal therapy (PTT) has aroused extensive attention in biomedical research recently because it can provide more comprehensive information for accurate diagnosis and treatment. In this research, the manganese ion chelated endogenous biopolymer melanin nanoparticles were successfully prepared for magnetic resonance (MR)/photoacoustic (PA) dual-modal imaging-guided PTT. The obtained nanoparticles with an ultrasmall size of about 3.2 nm exhibited negligible cytotoxicity, high relaxivity for MRI, an excellent photothermal effect and PA activity. Moreover, in vivo MRI and PAI results all demonstrated that the nanoparticles began to diffuse in the blood after intratumoral injection into tumor-bearing mice and could spread throughout the whole tumor region at 3 h, indicating the optimal treatment time. The subsequent photothermal therapy of cancer cells in vivo was carried out and the result showed that tumor growth could be effectively inhibited without inducing any observed side effects. Besides, melanin as an endogenous biopolymer has native biocompatibility and biodegradability, and it can be excreted through both renal and hepatobiliary pathways after treatment. Therefore, the melanin-Mn nanoparticles may assist in better indicating the optimal treatment time, monitoring the therapeutic process and enhancing the therapeutic effect and showed great clinical translation potential for cancer diagnosis and therapy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nanoscale Royal Society of Chemistry

Ultrasmall endogenous biopolymer nanoparticles for magnetic resonance/photoacoustic dual-modal imaging-guided photothermal therapyElectronic supplementary information (ESI) available: FT-IR spectra of MNP, PEG-MNP and NH2-PEG5000-NH2 (Fig. S1). Relationship of MNP-PEG with the amine group determined by fluorescamine (Fig. S2). Mn2+ stability study of MNP-Mn in PBS (pH = 7.4) and acidic buffer solution (pH = 5.5) (Fig. S3). Fig. S4 (a) photos of MNP-Mn in PBS and serum with different incubation time. (b) Hydrodynamic size distribution of the MNP-Mn during different incubation periods with serum, suggesting good stability in serum. Temperature curves of MNP-Mn with the same concentration (200 g mL1) and PBS irradiated by an 808 nm laser at different laser power density for 5 min (Fig. S5). Fig. S6 (a) temperature curves of MNP-Mn, ICG solutions and water under five cycles of photothermal heating by an 808 nm laser. (b) Photos of MNP-Mn, ICG solutions and water before and after photothermal heating. Linear time data versus ln() obtained from the cooling period (Fig. S7). Cell viabilities of the NIH 3T3 cells after 24 h incubation with MNP-Mn at various concentrations by MTT assay (Fig. S8). UV-Vis and fluorescence spectra of rhodamine and MNP-Mn-rhodamine solutions (Fig. S9). T1-Weighted MR coronal images after i.t. injection into the tumor bearing mice at 24 h and 5 day using 3.0 T clinical MRI equipment (Fig. S10). See DOI: 10.1039/c8nr01215f

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Publisher
Royal Society of Chemistry
Copyright
This journal is © The Royal Society of Chemistry
ISSN
2040-3364
D.O.I.
10.1039/c8nr01215f
Publisher site
See Article on Publisher Site

Abstract

Multi-modal imaging-guided photothermal therapy (PTT) has aroused extensive attention in biomedical research recently because it can provide more comprehensive information for accurate diagnosis and treatment. In this research, the manganese ion chelated endogenous biopolymer melanin nanoparticles were successfully prepared for magnetic resonance (MR)/photoacoustic (PA) dual-modal imaging-guided PTT. The obtained nanoparticles with an ultrasmall size of about 3.2 nm exhibited negligible cytotoxicity, high relaxivity for MRI, an excellent photothermal effect and PA activity. Moreover, in vivo MRI and PAI results all demonstrated that the nanoparticles began to diffuse in the blood after intratumoral injection into tumor-bearing mice and could spread throughout the whole tumor region at 3 h, indicating the optimal treatment time. The subsequent photothermal therapy of cancer cells in vivo was carried out and the result showed that tumor growth could be effectively inhibited without inducing any observed side effects. Besides, melanin as an endogenous biopolymer has native biocompatibility and biodegradability, and it can be excreted through both renal and hepatobiliary pathways after treatment. Therefore, the melanin-Mn nanoparticles may assist in better indicating the optimal treatment time, monitoring the therapeutic process and enhancing the therapeutic effect and showed great clinical translation potential for cancer diagnosis and therapy.

Journal

NanoscaleRoyal Society of Chemistry

Published: May 29, 2018

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