Fourier transform infrared (FTIR) spectroscopy has been considered as a powerful tool for analysing the characteristics of deoxyribonucleic acid (DNA) regardless of physical states, sample amounts and the molecular weight of DNA. However, FTIR spectroscopic analysis of DNA might be influenced by the stability and conformational integrity of deoxyribonucleic acid. This work systematically investigated the key factors in FTIR spectroscopic analysis of DNA and explored the influence of FTIR acquisition parameters, including FTIR sampling techniques (traditional KBr-FTIR, high throughput (HT)-FTIR, Micro-FTIR-point, and Micro-FTIR-image), pretreatment temperature, and sample concentration, on calf thymus DNA (CT-DNA). The results showed that the FTIR sampling techniques had a significant influence on the spectral characteristics, spectral quality, and sampling efficiency. Furthermore, HT-FTIR technique enabled rapid automated collection of spectra and was selected for obtaining better spectral accuracy and repeatability. Additionally, a pretreatment temperature of 30 C was selected to dry DNA, because the continuous increase of temperature had a great impact on the spectral analysis of DNA. The sample concentration was also proven to have a great effect on the spectral repeatability and SNR, especially for the initial sample amounts, where optimum sample amounts (100 g) were selected. These fundamental studies provide proof of a spectral collection that further supports subtle DNA structural analysis by FTIR spectroscopy.
Analytical Methods – Royal Society of Chemistry
Published: Apr 26, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera