In this study, we developed a microfluidic chip integrated with on-chip valves for quantitative detection of biomarkers. The sandwich-structured microfluidic chip consists of a top fluidic layer embedded with a zigzag channel, a middle tinfoil layer pre-patterned with protein stripes, and a bottom substrate layer with two waste chambers. A set of customized on-chip valves was introduced for precise control of fluid flow. To improve the production volume and uniformity among chips, we selected an injection molding method for mass-production of the main components. We standardized the process of sealing chip layers and assembling the valve holder to improve the effectiveness of cooperation with the instrument. To demonstrate the versatility of the microfluidic chip for quantitative detection of biomarkers, we used it to automatically detect C-reactive proteins (CRP), interleukin-6 (IL-6), insulin, and osteocalcin with high sensitivity and excellent reproducibility inside a customized and portable instrument. We further conducted the automated chip-based chemiluminescence immunoassay to test CRP in serum samples for demonstration of potential clinical applications, assess the activity of capture antibodies of CRP (CRP-Ab1) on the tinfoil layer for preliminary assessment of storage stability, and simultaneously detect CRP and IL-6 for confirmation of scalable multiplexation. This microfluidic chip for quantitative, automated, and portable immunoassay provides a promising choice for clinical practices.
Analytical Methods – Royal Society of Chemistry
Published: May 17, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera