A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegansElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ay00396c

A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine... Endocannabinoids and related N-acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans, several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as well as aging. One such molecule oleoylethanolamine (OEA) has been shown to promote life extension through nuclear receptor signal transduction, and its accurate quantitation therefore is of high interest. Using a combination of electron impact ionization (EI) and collision induced dissociation coupled to gas chromatography (GC), we found unique fragmentation ions of OEA and designed a specific MRM method for its accurate quantification. Our method should provide a reproducible and robust way to measure OEA dynamics under different genetic, pharmacologic and environmental perturbations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Methods Royal Society of Chemistry

A novel EI-GC/MS method for the accurate quantification of anti-aging compound oleoylethanolamine in C. elegansElectronic supplementary information (ESI) available. See DOI: 10.1039/c8ay00396c

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Publisher
The Royal Society of Chemistry
Copyright
This journal is © The Royal Society of Chemistry
ISSN
1759-9660
eISSN
1759-9679
D.O.I.
10.1039/c8ay00396c
Publisher site
See Article on Publisher Site

Abstract

Endocannabinoids and related N-acyl ethanolamine-derived lipids affect a diverse array of physiological processes and pathological conditions. In the roundworm C. elegans, several endocannabinoid-like molecules have been implicated in regulating axon regeneration, energy balance and food intake as well as aging. One such molecule oleoylethanolamine (OEA) has been shown to promote life extension through nuclear receptor signal transduction, and its accurate quantitation therefore is of high interest. Using a combination of electron impact ionization (EI) and collision induced dissociation coupled to gas chromatography (GC), we found unique fragmentation ions of OEA and designed a specific MRM method for its accurate quantification. Our method should provide a reproducible and robust way to measure OEA dynamics under different genetic, pharmacologic and environmental perturbations.

Journal

Analytical MethodsRoyal Society of Chemistry

Published: Apr 30, 2018

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