A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodesElectronic supplementary information (ESI) available: Experimental description; schematic representation of the experimental setup for neuronal stimulation experiments (and controls); in vitro ACh detection in ASW background solution with the calibration curve; cyclic voltammograms of components of ASW solution; COMSOL simulation of the pipet approach and experimental SECM approach curve to the neuronal soma surface; quantitative and kinetic parameters of release events; control experiments to confirm somatic release instead of physical disturbance that could occur during the stimulation process; results of control experiments demonstrating probe selectivity against stimulating solution; and distance dependence results on acetylcholine somatic release, before and after nano-positioning with an SECM. See DOI: 10.1039/c8sc01131a

A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and... Extra-synaptic exocytosis is an essential component of cellular communication. A knowledge gap exists in the exocytosis of the non-redox active transmitter acetylcholine. Using the nano-interface between two immiscible electrolyte solutions and scanning electrochemical microscopy (SECM), a high resolution spatiotemporal study of acetylcholine exocytosis is shown from an individual neuronal soma. The nanoelectrode was positioned 140 nm away from the release sites on the soma using an SECM. The quantitative study enables the obtaining of key information related to cellular communication, including extracellular concentration of the neurotransmitter, cellular permeability, Ca2+ dependence on somatic release, vesicle density, number of molecules released and the release dynamics. Measurements were achieved with a high signal to noise ratio of 619. The released neurotransmitter with a concentration of 2.7 (1.0) M was detected at the nanoelectrodes with radii of 750 nm to 860 nm. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chemical Science Royal Society of Chemistry

A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodesElectronic supplementary information (ESI) available: Experimental description; schematic representation of the experimental setup for neuronal stimulation experiments (and controls); in vitro ACh detection in ASW background solution with the calibration curve; cyclic voltammograms of components of ASW solution; COMSOL simulation of the pipet approach and experimental SECM approach curve to the neuronal soma surface; quantitative and kinetic parameters of release events; control experiments to confirm somatic release instead of physical disturbance that could occur during the stimulation process; results of control experiments demonstrating probe selectivity against stimulating solution; and distance dependence results on acetylcholine somatic release, before and after nano-positioning with an SECM. See DOI: 10.1039/c8sc01131a

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Publisher
Royal Society of Chemistry
Copyright
This journal is © The Royal Society of Chemistry
ISSN
2041-6520
D.O.I.
10.1039/c8sc01131a
Publisher site
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Abstract

Extra-synaptic exocytosis is an essential component of cellular communication. A knowledge gap exists in the exocytosis of the non-redox active transmitter acetylcholine. Using the nano-interface between two immiscible electrolyte solutions and scanning electrochemical microscopy (SECM), a high resolution spatiotemporal study of acetylcholine exocytosis is shown from an individual neuronal soma. The nanoelectrode was positioned 140 nm away from the release sites on the soma using an SECM. The quantitative study enables the obtaining of key information related to cellular communication, including extracellular concentration of the neurotransmitter, cellular permeability, Ca2+ dependence on somatic release, vesicle density, number of molecules released and the release dynamics. Measurements were achieved with a high signal to noise ratio of 619. The released neurotransmitter with a concentration of 2.7 (1.0) M was detected at the nanoelectrodes with radii of 750 nm to 860 nm.

Journal

Chemical ScienceRoyal Society of Chemistry

Published: May 24, 2018

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