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THE ISOLATION OF WHEAT GERM NUCLEI AND SOME ASPECTS OF THEIR GLYCOLYTIC METABOLISM

THE ISOLATION OF WHEAT GERM NUCLEI AND SOME ASPECTS OF THEIR GLYCOLYTIC METABOLISM 1. A procedure for isolating nuclei of the wheat germ in non-aqueous media has been described. 2. Such nuclei were shown to constitute about 50 per cent of the protoplasmic mass and to have a ribonucleic acid content of an order equivalent to that of the cytoplasm. 3. Studies of the distribution of the enzymes—aldolase, phosphoglyceraldehyde dehydrogenase, enolase, and pyruvate kinase—have revealed that the nuclei are the most vigorous sites of glycolytic activity. 4. Analysis of the DPN content of the nuclei in calf tissues—liver, pancreas, and heart—pointed to the probability that glycolytic activity is a characteristic common to many nuclei. 5. The significance of glycolytic activity to nuclear function has been discussed and some suggestive comparisons made between the two energy-yielding systems of glycolytic and oxidative phosphorylation. Footnotes Submitted: 2 July 1952 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of General Physiology Rockefeller University Press

THE ISOLATION OF WHEAT GERM NUCLEI AND SOME ASPECTS OF THEIR GLYCOLYTIC METABOLISM

The Journal of General Physiology , Volume 36 (2): 181 – Nov 20, 1952

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Publisher
Rockefeller University Press
Copyright
© 1952 Rockefeller University Press
ISSN
0022-1295
eISSN
1540-7748
DOI
10.1085/jgp.36.2.181
Publisher site
See Article on Publisher Site

Abstract

1. A procedure for isolating nuclei of the wheat germ in non-aqueous media has been described. 2. Such nuclei were shown to constitute about 50 per cent of the protoplasmic mass and to have a ribonucleic acid content of an order equivalent to that of the cytoplasm. 3. Studies of the distribution of the enzymes—aldolase, phosphoglyceraldehyde dehydrogenase, enolase, and pyruvate kinase—have revealed that the nuclei are the most vigorous sites of glycolytic activity. 4. Analysis of the DPN content of the nuclei in calf tissues—liver, pancreas, and heart—pointed to the probability that glycolytic activity is a characteristic common to many nuclei. 5. The significance of glycolytic activity to nuclear function has been discussed and some suggestive comparisons made between the two energy-yielding systems of glycolytic and oxidative phosphorylation. Footnotes Submitted: 2 July 1952

Journal

The Journal of General PhysiologyRockefeller University Press

Published: Nov 20, 1952

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