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THE DISTRIBUTION OF INORGANIC CATIONS DURING SPERMIOGENESIS IN THE MOUSE

THE DISTRIBUTION OF INORGANIC CATIONS DURING SPERMIOGENESIS IN THE MOUSE Inorganic cations have been localized in several animal and plant tissues by means of the pyroantimonate fixation procedure (1-3) . We demonstrated that an aqueous solution of potassium pyroantimonate enters the cell without disruption of cell membranes and produces electron-opaque cation-antimonate precipitates in a constant and reproducible manner . The results obtained in mouse testis (3) were further extended to the meiotic prophase in spermatogenesis and to Sertoli's cells .' In the present paper we studied the distribution of pyroantimonate-precipitable cations during spermiogenesis . We demonstrate regional differences with respect to the occurrence of the precipitate of the spermatid during evolution . MATERIALS AND METHODS Adult Swiss mice (Rockland strain) were anesthetized with ether . The testes were fixed in a saturated solution of potassium pyroantimonate (Riedel-De Haen AG, Seelze, Hannover, Germany), pH about 9 .2, hardened with formaldehyde, and postosmicated as described before (3) . 1 The sections were examined unstained . Staining of pyroantimonate-fixed sections was accomplished by first immersing the sections in a dilute solution of oxalic acid (1 vol of a saturated aqueous solution in 400 vol of distilled water), washing with distilled water, and then staining with uranyl acetate and lead citrate as http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

THE DISTRIBUTION OF INORGANIC CATIONS DURING SPERMIOGENESIS IN THE MOUSE

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Publisher
Rockefeller University Press
Copyright
Copyright © 1972 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.53.1.239
Publisher site
See Article on Publisher Site

Abstract

Inorganic cations have been localized in several animal and plant tissues by means of the pyroantimonate fixation procedure (1-3) . We demonstrated that an aqueous solution of potassium pyroantimonate enters the cell without disruption of cell membranes and produces electron-opaque cation-antimonate precipitates in a constant and reproducible manner . The results obtained in mouse testis (3) were further extended to the meiotic prophase in spermatogenesis and to Sertoli's cells .' In the present paper we studied the distribution of pyroantimonate-precipitable cations during spermiogenesis . We demonstrate regional differences with respect to the occurrence of the precipitate of the spermatid during evolution . MATERIALS AND METHODS Adult Swiss mice (Rockland strain) were anesthetized with ether . The testes were fixed in a saturated solution of potassium pyroantimonate (Riedel-De Haen AG, Seelze, Hannover, Germany), pH about 9 .2, hardened with formaldehyde, and postosmicated as described before (3) . 1 The sections were examined unstained . Staining of pyroantimonate-fixed sections was accomplished by first immersing the sections in a dilute solution of oxalic acid (1 vol of a saturated aqueous solution in 400 vol of distilled water), washing with distilled water, and then staining with uranyl acetate and lead citrate as

Journal

The Journal of Cell Biology – Rockefeller University Press

Published: Apr 1, 1972

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