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The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster

The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic... Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis. Footnotes Abbreviations used in this paper: CNS, central nervous system; D-TACC, Drosophila melanogaster– transforming acidic coiled coil; msps , mini spindles ; NHK-1, nucleosomal histone kinase-1; RNAi, RNA interference; SNP, single nucleotide polymorphism. Submitted: 19 August 2005 Accepted: 18 October 2005 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster

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Publisher
Rockefeller University Press
Copyright
© 2005 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.200508127
pmid
16301329
Publisher site
See Article on Publisher Site

Abstract

Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis. Footnotes Abbreviations used in this paper: CNS, central nervous system; D-TACC, Drosophila melanogaster– transforming acidic coiled coil; msps , mini spindles ; NHK-1, nucleosomal histone kinase-1; RNAi, RNA interference; SNP, single nucleotide polymorphism. Submitted: 19 August 2005 Accepted: 18 October 2005

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Nov 21, 2005

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