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Conventional centrosomes are absent from the spindle in female meiosis in many species, but it is not clear how multiple chromosomes form one shared bipolar spindle without centrosomes. We identified a female sterile mutant in which each bivalent chromosome often forms a separate bipolar metaphase I spindle. Unlike wild type, prophase I chromosomes fail to form a single compact structure within the oocyte nucleus, although the integrity of metaphase I chromosomes appears to be normal. Molecular analysis indicates that the mutant is defective in the conserved kinase nucleosomal histone kinase-1 (NHK-1). Isolation of further alleles and RNA interference in S2 cells demonstrated that NHK-1 is also required for mitotic progression. NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis. Footnotes Abbreviations used in this paper: CNS, central nervous system; D-TACC, Drosophila melanogaster– transforming acidic coiled coil; msps , mini spindles ; NHK-1, nucleosomal histone kinase-1; RNAi, RNA interference; SNP, single nucleotide polymorphism. Submitted: 19 August 2005 Accepted: 18 October 2005
The Journal of Cell Biology – Rockefeller University Press
Published: Nov 21, 2005
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