Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Trinkaus (1984)
Cells into Organs: The Forces That Shape the Embryo
N. Neff, C. Lowrey, C. Decker, A. Tovar, C. Damsky, C. Buck, A. Horwitz (1982)
A monoclonal antibody detaches embryonic skeletal muscle from extracellular matricesThe Journal of Cell Biology, 95
A. Chapman (1984)
Characterization of a 140Kd cell surface glycoprotein involved in myoblast adhesionJournal of Cellular Biochemistry, 25
R. Timpl, J. Engel, G. Martin (1983)
Laminin — a multifunctional protein of basement membranesTrends in Biochemical Sciences, 8
J. Couchman, M. Hook, D. Rees, R. Timpl (1983)
Adhesion, growth, and matrix production by fibroblasts on laminin substratesThe Journal of Cell Biology, 96
D. McClay, G. Wessel, R. Marchase (1981)
Intercellular recognition: quantitation of initial binding events.Proceedings of the National Academy of Sciences of the United States of America, 78 8
J. Greve, D. Gottlieb (1982)
Monoclonal Antibodies Which Alter the Morphology of Cultured Chick Myogenic CellsJournal of Cellular Biochemistry, 18
S. Rogers, J. McCarthy, S. Palm, L. Furcht, Paul Letourneau (1985)
Neuron-specific interactions with two neurite-promoting fragments of fibronectin, 5
(1984)
Interaction of cells with extracellular matrix components
S. Clarke (1975)
The size and detergent binding of membrane proteins.The Journal of biological chemistry, 250 14
S. Akiyama, K. Yamada (1985)
The interaction of plasma fibronectin with fibroblastic cells in suspension.The Journal of biological chemistry, 260 7
H. Kleinman, F. Cannon, G. Laurie, J. Hassell, M. Aumailley, V. Terranova, G. Martin, M. Dubois‐Dalcq (1985)
Biological activities of lamininJournal of Cellular Biochemistry, 27
M. Pierschbacher, E. Ruoslahti (1984)
Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the moleculeNature, 309
(1978)
Cell-to-substrate contacts of chick fibroblasts and their relation to the microfilament system. A correlated interference reflection and high voltage electron microscope study
T. Hasegawa, E. Hasegawa, Wen‐Tien Chen, Kenneth Yamada (1985)
Characterization of a membrane‐associated glycoprotein complex implicated in cell adhesion to fibronectinJournal of Cellular Biochemistry, 28
(1983)
Isolation of a cell surface receptor for laminin from murine sarcoma cells
W. Chen, J. Greve, D. Gottlieb, S. Singer (1985)
Immunocytochemical localization of 140 kD cell adhesion molecules in cultured chicken fibroblasts, and in chicken smooth muscle and intestinal epithelial tissues.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33
(1985)
The participation of a putative fibronectin and laminin receptor in neurite extension
R. Bloch, Z. Hall (1983)
Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filaminThe Journal of Cell Biology, 97
G. Tarone, G. Tarone, G. Galetto, G. Galetto, M. Prat, M. Prat, P. Comoglio, P. Comoglio (1982)
Cell surface molecules and fibronectin-mediated cell adhesion: effect of proteolytic digestion of membrane proteinsThe Journal of Cell Biology, 94
J. Cell Biol
(1985)
Distribution of HORWfTZ ET AL. A Laminin and Fibronectin Receptor cell substratum attachment (CSAT) antigen on myogenic and fibroblastic cells in culture
D. Edgar, R. Timpl, H. Thoenen (1984)
The heparin‐binding domain of laminin is responsible for its effects on neurite outgrowth and neuronal survival.The EMBO Journal, 3
Karen Knudsen, Alan Horwitz, Clayton Buck (1985)
A monoclonal antibody identifies a glycoprotein complex involved in cell-substratum adhesion.Experimental cell research, 157 1
M. Daniels, M. Vigny, P. Sonderegger, H. Bauer, Z. Vogel (1984)
Association of laminin and other basement membrane components with regions of high acetylcholine receptor density on cultured myotubesInternational Journal of Developmental Neuroscience, 2
V. Terranova, C. Rao, T. Kalebic, I. Margulies, L. Liotta (1983)
Laminin receptor on human breast carcinoma cells.Proceedings of the National Academy of Sciences of the United States of America, 80 2
W. Chen, E. Hasegawa, T. Hasegawa, C. Weinstock, K. Yamada (1985)
Development of cell surface linkage complexes in cultured fibroblastsThe Journal of Cell Biology, 100
M. Pierschbacher, E. Ruoslahti (1984)
Variants of the cell recognition site of fibronectin that retain attachment-promoting activity.Proceedings of the National Academy of Sciences of the United States of America, 81 19
N. Rao, S. Barsky, V. Terranova, L. Liotta (1983)
Isolation of a tumor cell laminin receptor.Biochemical and biophysical research communications, 111 3
L. Liotta, P. Hand, C. Rao, G. Bryant, S. Barsky, J. Schlom (1985)
Monoclonal antibodies to the human laminin receptor recognize structurally distinct sites.Experimental cell research, 156 1
(1981)
Manipulation of cell - cell and cell - substratum interaction in mouse mammary tumor epithelia
A. Fersht (1977)
Enzyme structure and mechanism
T. Haga, K. Haga, A. Gilman (1977)
Hydrodynamic properties of the beta-adrenergic receptor and adenylate cyclase from wild type and varient S49 lymphoma cells.The Journal of biological chemistry, 252 16
C. Izzard, L. Lochner (1976)
Cell-to-substrate contacts in living fibroblasts: an interference reflexion study with an evaluation of the technique.Journal of cell science, 21 1
(1982)
Immunoclectron microscopic studies of the sites of cell-substratum and cell-cell contact in culture fibroblasB
R. Pytela, M. Pierschbacher, E. Ruoslahti (1985)
Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptorCell, 40
C. Decker, R. Greggs, K. Duggan, J. Stubbs, A. Horwitz (1984)
Adhesive multiplicity in the interaction of embryonic fibroblasts and myoblasts with extracellular matricesThe Journal of Cell Biology, 99
(1985)
Selective inhibition of fibronectin mediated adhesion by monoclonai antibodies
H. Lesot, U. Kühl, K. Mark (1983)
Isolation of a laminin‐binding protein from muscle cell membranesThe EMBO Journal, 2
R. Martin, B. Ames (1961)
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.The Journal of biological chemistry, 236
C. Tanford, J. Reynolds (1976)
Characterization of membrane proteins in detergent solutions.Biochimica et biophysica acta, 457 2
J. Hummel, W. Dreyer (1962)
Measurement of protein-binding phenomena by gel filtration.Biochimica et biophysica acta, 63
A. Horwitz, K. Knudsen, C. Damsky, C. Decker, C. Buck, N. Neff (1984)
Adhesion-Related Integral Membrane Glycoproteins Identified by Monoclonal Antibodies
(1982)
Fibronectins: a molecule with remarkable structural and functional diversity
R. Hynes, K. Yamada (1982)
Fibronectins: multifunctional modular glycoproteinsThe Journal of Cell Biology, 95
K. Knudsen, P. Rao, C. Damsky, C. Buck (1981)
Membrane glycoproteins involved in cell--substratum adhesion.Proceedings of the National Academy of Sciences of the United States of America, 78 10
R. Zahler (1979)
Enzyme Structure and MechanismThe Yale Journal of Biology and Medicine, 52
The cell substrate attachment (CSAT) antigen is an integral membrane glycoprotein complex that participates in the adhesion of cells to extracellular molecules. The CSAT monoclonal antibody, directed against this complex, inhibited adhesion of cardiac and tendon fibroblasts and skeletal myoblasts to both laminin and fibronectin, thus implicating the CSAT antigen in adhesion to these extracellular molecules. Equilibrium gel filtration was used to explore the hypothesis that the CSAT antigen functions as a cell surface receptor for both laminin and fibronectin. In this technique, designed for rapidly exchanging equilibria, the gel filtration column is pre-equilibrated with extracellular ligand to ensure receptor occupancy during its journey through the column. Both laminin and fibronectin formed complexes with the CSAT antigen. The association with laminin was inhibited by the CSAT monoclonal antibody; the associations with both fibronectin and laminin were inhibited by synthetic peptides containing the fibronectin cell-binding sequence. Estimates of the dissociation constants by equilibrium gel filtration agree well with those available from other measurements. This suggests that these associations are biologically significant. SDS PAGE showed that all three glycoproteins comprising the CSAT antigen were present in the antigen-ligand complexes. Gel filtration and velocity sedimentation were used to show that the three bands comprise and oligomeric complex, which provides an explanation for their functional association. The inhibition of adhesion by the CSAT monoclonal antibody and the association of the purified antigen with extracellular ligands are interpreted as strongly implicating the CSAT antigen as a receptor for both fibronectin and laminin and perhaps for other extracellular molecules as well.
The Journal of Cell Biology – Rockefeller University Press
Published: Dec 1, 1985
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.